Deciphering the transcriptomic response of Fusarium verticillioides in relation to nitrogen availability and the development of sugarcane pokkah boeng disease.
Deciphering the transcriptomic response of Fusarium verticillioides in relation to nitrogen availability and the development of sugarcane pokkah boeng disease.
复制标题
破译轮枝镰刀菌与氮利用率和甘蔗 pokkah boeng 病发展相关的转录组学反应
DOI：
10.1038/srep29692
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发表时间：
2016-07-20
影响因子：
4.6
通讯作者：
Zhang M
中科院分区：
文献类型：
--
作者：
Lin Z;Wang J;Bao Y;Guo Q;Powell CA;Xu S;Chen B;Zhang M
由轮枝镰刀菌（Fusarium verticillioides）引起的甘蔗黑穗病（Pokkah boeng）是甘蔗生产上的一种严重病害。病害的严重程度与甘蔗基因型以及环境因素如施氮有关。研究了氮源（硫酸铵、尿素或硝酸钠）对甘蔗薄壳病及其病原菌的影响，并在体外测定了真菌生长和产孢量。结果表明，与尿素相比，铵态氮和硝态氮有利于甘蔗白叶枯病菌菌丝生长、细胞密度和产孢量的增加，从而加重了甘蔗白叶枯病的症状。从全局转录组学分析鉴定的13，999个注释基因中，共有1，779个转录本在F.轮枝孢CNO-1在不同氮源条件下的生长情况。这些被发现参与氮代谢，运输和同化。基于PHI-base数据库，这些基因中的许多也与致病性相关。一些转录因子被发现与特定的生物过程有关的氮利用。我们的研究结果进一步表明，氮的可用性可能在疾病的发展中发挥重要作用，通过增加真菌细胞的生长，以及影响成功的发病所需的基因的表达。
Pokkah boeng, caused by Fusarium verticillioides, is a serious disease in sugarcane industry. The disease severity is related to the sugarcane genotype as well as environmental considerations, such as nitrogen application. The impact of the nitrogen source (ammonium sulfate, urea, or sodium nitrate) on sugarcane pokkah boeng disease and its pathogen was investigated in planta and fungal growth and sporulation production was measured in vitro. The results showed that ammonium and nitrate were beneficial to fungal mycelium growth, cell densities and sporulation, which enhanced the disease symptoms of sugarcane pokkah boeng compared to urea fertilization. A total of 1,779 transcripts out of 13,999 annotated genes identified from global transcriptomic analysis were differentially expressed in F. verticillioides CNO-1 grown in the different sources of nitrogen. These were found to be involved in nitrogen metabolism, transport and assimilation. Many of these genes were also associated with pathogenicity based on the PHI-base database. Several transcription factors were found to be associated with specific biological processes related to nitrogen utilization. Our results further demonstrated that nitrogen availability might play an important role in disease development by increasing fungal cell growth as well as influencing the expression of genes required for successful pathogenesis.