Poly(2-aminoethyl methacrylate)-based polyampholyte brush surface with carboxylic groups to improve blood compatibility
Poly(2-aminoethyl methacrylate)-based polyampholyte brush surface with carboxylic groups to improve blood compatibility
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DOI：
10.1080/09205063.2019.1710900
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发表时间：
2019-12
期刊：
影响因子：
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通讯作者：
Tomoyuki Azuma;T. Matsushita;V. Manivel;K. Nilsson Ekdahl;B. Nilsson;Y. Teramura;M. Takai
中科院分区：
文献类型：
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作者：
Tomoyuki Azuma;T. Matsushita;V. Manivel;K. Nilsson Ekdahl;B. Nilsson;Y. Teramura;M. Takai
摘要基于两性离子材料的聚合物刷可显著抑制蛋白质吸附和细胞粘附，从而提高血液相容性。然而，两性离子聚合物本身难以进一步改性，因为改性后电荷平衡受损，从而影响血液相容性。本研究设计了一种可化学修饰的混合电荷聚合物刷，而不影响其特性。凝聚的混合电荷聚合物刷将像两性离子材料一样工作，因为据报道相邻的相反电荷在两性离子材料中是重要的。以甲基丙烯酸2-氨基乙酯（AEMA）为原料，合成了带伯胺基团的阳离子聚合物刷，并用丁二酸酐对其进行改性，得到羧基引发的聚（AEMA）。优化了伯胺基和羧基的比例，得到了两性聚电解质刷。通过测量聚合物诱导的凝血/补体激活、蛋白质吸附和细胞粘附来评价血液相容性。我们设计的基于阳离子的聚两性电解质刷防止凝血/补体激活与聚（2-甲基丙烯酰氧基乙基磷酰胆碱）刷相当，基于单体内相互作用，因为凝聚的混合电荷像两性离子一样起作用。
Abstract Zwitterionic material-based polymer brush significantly prevents protein adsorption and cell adhesion, which leads to the blood compatibility. However, zwitterionic polymer itself is difficult to be modified further, for the blood compatibility since the charged balance is impaired after the modification. In this research, chemically modifiable mixed charge polymer brush is designed, without impairing its characteristics. Condensed mixed charge polymer brush will work like zwitterionic material because neighbouring opposite charge is reported to be important in the zwitterionic material. Cationic polymer brush with primary amine group, which is based on 2-aminoethyl methacrylate (AEMA), was prepared and modified by succinic anhydride to obtain carboxylic group induced poly(AEMA). The ratio of primary amine group and carboxylic group was optimized to obtain the polyampholyte brush. The blood compatibility was evaluated by measuring coagulation/complement activation, protein adsorption and cell adhesion induced by the polymer. Our designed cationic-based polyampholyte brush prevented coagulation/complement activation comparable to poly(2-methacryloyloxyethyl phosphorylcholine) brush, based on intra-monomer interaction, because condensed mix charge works like zwitterion.