Triterpene-enriched extracts from Ganoderma lucidum inhibit growth of hepatoma cells via suppressing protein kinase C, activating mitogen-activated protein kinases and G2-phase cell cycle arrest
Triterpene-enriched extracts from Ganoderma lucidum inhibit growth of hepatoma cells via suppressing protein kinase C, activating mitogen-activated protein kinases and G2-phase cell cycle arrest
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DOI：
10.1016/s0024-3205(03)00124-3
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发表时间：
2003-04-11
期刊：
影响因子：
6.1
通讯作者：
Kan, LS
中科院分区：
文献类型：
--
作者：
Lin, SB;Li, CH;Kan, LS
药用灵芝(灵芝)在东方已被用于预防和治疗包括癌症在内的各种疾病。除了其多糖成分的免疫增强特性外，人们对另一种主要成分三萜类化合物的抗癌活性知之甚少。本文研究了灵芝富含三萜提取物的抗癌作用机制。以灵芝菌丝体为原料制备了富含三萜的组分WEES-G6。通过热水连续提取，除去乙醇不溶性多糖，然后进行凝胶过滤层析。我们发现WEES-G6抑制人肝癌Huh-7细胞的生长，但不抑制人正常肝细胞系Chang肝细胞的生长。经WeES-G6处理后，细胞生长调节蛋白PKC活性迅速下降，JNK和p38MAP激酶活性迅速降低，这些分子的变化导致G2期延长，对细胞生长的抑制作用较强。在正常肝细胞中没有观察到这些影响。我们的发现表明，灵芝中所含的三萜类化合物是潜在的抗癌药物。(C)2003 Elsevier Science Inc.保留所有权利。
The medicinal mushroom Ganoderma lucidum (G. lucidum) has been used in the Orient for the prevention and treatment of various diseases including cancer. Except for the immune enhancing properties of its polysaccharide constituent, very little is known about the anticancer activity of another major constituent, triterpenes. In this report, we studied the anticancer mechanism of triterpene-enriched extracts from G. lucidum. The triterpene-enriched fraction, WEES-G6, was prepared from mycelia of G. lucidum. by sequential hot water extraction, removal of ethanol-insoluble polysaccharides and then, gel-filtration chromatography. We found that WEES-G6 inhibited growth of human hepatoma Huh-7 cells, but not Chang liver cells, a normal human liver cell line. Treatment with WEES-G6 caused a rapid decrease in the activity of cell growth regulative protein, PKC, and the activation of JNK and p38 MAP kinases, The changes in these molecules resulted in a prolonged G2 cell cycle phase and strong growth inhibition. None of these effects were seen in the normal liver cells. Our findings suggest that the triterpenes contained in G. lucidum are potential, anticancer agents.' (C) 2003 Elsevier Science Inc. All rights reserved.