Drosophila E-cadherin is essential for proper germ cell-soma interaction during gonad morphogenesis
Drosophila E-cadherin is essential for proper germ cell-soma interaction during gonad morphogenesis
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DOI：
10.1242/dev.00639
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发表时间：
2003-09-01
期刊：
影响因子：
4.6
通讯作者：
Van Doren, M
中科院分区：
文献类型：
--
作者：
Jenkins, AB;McCaffery, JM;Van Doren, M
在大多数动物物种中，生殖细胞需要与性腺中的特化体细胞密切接触才能正常发育。我们分析了果蝇胚胎性腺形成过程中生殖细胞-体细胞相互作用的建立，发现体细胞在细胞形状上发生了巨大的变化，并在性腺合并时单独包裹生殖细胞。生殖细胞鞘是独立的性腺形成的其他方面，表明单独的形态发生过程中的工作在性腺发生。细胞-细胞粘附分子果蝇E-钙粘蛋白是必不可少的两个生殖细胞ensheathment和性腺压实，并上调体细胞性腺在性腺形成的时间。我们的数据表明，差异细胞粘附有助于细胞分选和形成适当的性腺结构。此外，我们还发现，一种新的跨膜蛋白，对亲密的恐惧，也需要两个生殖细胞鞘和性腺压缩。性腺中的E-钙粘蛋白表达在亲密恐惧突变体中显著降低，表明亲密恐惧可能是E-钙粘蛋白表达或功能的调节剂。
In most animal species, germ cells require intimate contact with specialized somatic cells in the gonad for their proper development. We have analyzed the establishment of germ cell-soma interaction during embryonic gonad formation in Drosophila melanogaster, and find that somatic cells undergo dramatic changes in cell shape and individually ensheath germ cells as the gonad coalesces. Germ cell ensheathment is independent of other aspects of gonad formation, indicating that separate morphogenic processes are at work during gonadogenesis. The cell-cell adhesion molecule Drosophila E-cadherin is essential both for germ cell ensheathment and gonad compaction, and is upregulated in the somatic gonad at the time of gonad formation. Our data indicate that differential cell adhesion contributes to cell sorting and the formation of proper gonad architecture. In addition, we find that Fear of Intimacy, a novel transmembrane protein, is also required for both germ cell ensheathment and gonad compaction. E-cadherin expression in the gonad is dramatically decreased in fear of intimacy mutants, indicating that Fear of Intimacy may be a regulator of E-cadherin expression or function.