OXYGEN RADICAL INDUCED ALTERATIONS IN POLYCLONAL IGG
OXYGEN RADICAL INDUCED ALTERATIONS IN POLYCLONAL IGG
复制标题
DOI：
10.1016/0014-5793(88)80661-6
复制
发表时间：
1988-03-28
期刊：
影响因子：
3.5
通讯作者：
WILLSON, RL
中科院分区：
文献类型：
--
作者：
GRIFFITHS, HR;LUNEC, J;WILLSON, RL
在类风湿性关节炎患者的血清和滑液中，部分 IgG 部分以聚集和荧光形式存在。氧自由基与这种变性有关，尽管具体的自由基种类尚不清楚。在这项工作中，放射分解产生的氧自由基可以攻击溶液中的多克隆 IgG。 OH自由基诱导单体聚集，并在可见光区域出现新的荧光（Ex 360 nm，Em 454 nm）。发现超氧自由基阴离子在这两个方面都是惰性的，而过氧自由基诱导自发荧光而不伴随聚集。结果表明OH和/或过氧自由基攻击可能是IgG变性的体内机制。
In the sera and synovial fluid of patients with rheumatoid arthritis, part of the IgG fraction is found in an aggregated and fluorescent form. Oxygen-free radicals have been implicated in this denaturation, although the precise radical species responsible is unknown. In this work, oxygen-free radicals generated radiolytically were allowed to attack polyclonal IgG in solution. OH.radicals induced aggregation of the monomer and a new fluorescence appeared in the visible region (Ex 360 nm, Em 454 nm). The superoxide radical anion was found to be inert in both these respects, whilst peroxy radicals induced autofluorescence without concomitant aggregation. The results suggest that OH.and/or peroxy radical attack may be an in vivo mechanism for IgG denaturation.