Efficacy of garbanzo and soybean flour in suppression of aberrant crypt foci in the colons of CF-1 mice.
Efficacy of garbanzo and soybean flour in suppression of aberrant crypt foci in the colons of CF-1 mice.
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发表时间：
2004-09
影响因子：
2
通讯作者：
G. Murillo;Juliana K. Choi;Olivia Pan;A. Constantinou;R. Mehta
中科院分区：
文献类型：
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作者：
G. Murillo;Juliana K. Choi;Olivia Pan;A. Constantinou;R. Mehta
背景流行病学研究报告豆类消费量高的国家结肠癌发病率较低。此外，实验研究发现豆类，如大豆和斑豆，具有抗癌特性。虽然鹰嘴豆富含各种植物化学物质，但尚未得到充分研究。在本研究中，以CF-1小鼠中氧化偶氮甲烷（AOM）诱导的异常隐窝病灶（ACF）为模型来评估和比较鹰嘴豆粉与大豆粉的效果。材料和方法 将 20 只 5 周大的 CF-1 小鼠分为四组，每组 5 只动物：10% 鹰嘴豆、10% 大豆、10% 混合（大豆和鹰嘴豆粉）和对照（啮齿动物饲料）。动物每周一次皮下注射AOM（10mg/kg体重），持续两周以诱导ACF。第十周时，处死动物并对结肠进行评分。结果 饲喂鹰嘴豆粉的动物的 ACF 抑制率为 64%（p <0.001），而大豆和混合面粉组的 ACF 抑制率分别为 58% 和 55%（p<0.001）。讨论这些结果表明鹰嘴豆具有生物活性化合物，能够抑制小鼠癌前病变的形成，并表明与大豆一样，鹰嘴豆的食用有助于降低结肠癌的发病率。
BACKGROUND Epidemiological studies have reported a low incidence of colon cancer in countries with high legume consumption. Moreover, experimental studies have found that legumes, such as soybeans and pinto beans, have anticancer properties. While garbanzo beans are a rich source of various phytochemicals, they have not been well studied. In the present study, the azoxymethane (AOM)-induced aberrant crypt foci (ACF) in CF-1 mice was utilized as a model to assess and compare the effects of garbanzo flour to that of soy flour. MATERIALS AND METHODS Twenty, 5-week-old CF-1 mice were divided into four groups of 5 animals each: 10% garbanzo, 10% soy, 10% mixed (soy and garbanzo flours), and control (rodent chow). Animals received subcutaneous injections of AOM (10-mg/kg B. W.) once a week for two weeks to induce ACF. At week ten, the animals were sacrificed and the colons were scored. RESULTS There was a 64% (p <0.001) suppression of ACF for animals fed the garbanzo flour, versus an inhibition of 58 and 55% (p<0.001) for the soy and mixed flour groups, respectively. DISCUSSION These results demonstrate that garbanzo beans possess bioactive compounds capable of inhibiting the formation of pre-cancerous lesions in mice and suggest that, like soybeans, their consumption contributes to a reduction in colon cancer incidence.