Distribution and densitometry mapping of L1-CAM immunoreactivity in the adult mouse brain--light microscopic observation.
Distribution and densitometry mapping of L1-CAM immunoreactivity in the adult mouse brain--light microscopic observation.
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L1-CAM免疫反应性的分布和光密度分析图中的成年小鼠脑 - 光显微镜观察。
DOI：
10.1186/1471-2202-4-7
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发表时间：
2003-04-16
期刊：
影响因子：
2.4
通讯作者：
Shiosaka S
中科院分区：
文献类型：
--
作者：
Munakata H;Nakamura Y;Matsumoto-Miyai K;Itoh K;Yamasaki H;Shiosaka S
生理学和行为学研究表明，L1与海马区的可塑性和恐惧条件反射有关，表明L1在成熟大脑中表达的重要性。L1在小鼠脑内的分布可能为了解其在脑中的作用提供依据。我们使用两种针对L1不同表位的多克隆抗体，通过免疫组织化学方法检测了L1在成年小鼠脑中的总体分布。L1免疫反应阳性物质广泛而不均匀地分布于嗅球至上颈髓。L1免疫反应阳性物质主要分布于外侧嗅束、前连合的嗅束和颞肢、胼胝体、终纹、苍白球、穹隆、乳房丘脑束、孤束和三叉神经脊束的非神经元成分。非神经元和神经元L1在灰质，即梨状皮质和内嗅皮质、下丘脑、黑质网状部、中脑导水管周围灰质、三叉神经脊核等处有高到高水平的L1表达。L1神经元在嗅球、大脑皮层V层、杏仁核、桥脑灰质、上丘、小脑皮质、孤束核等部位有高到中等密度。神经元L1仅在海马区、尾壳核灰质、丘脑、小脑核等处有低到低水平的神经元L1表达。轴突、突触和细胞体，也存在于非神经元成分中。
The importance of L1 expression in the matured brain is suggested by physiological and behavioral studies showing that L1 is related to hippocampal plasticity and fear conditioning. The distribution of L1 in mouse brain might provide a basis for understanding its role in the brain. We examined the overall distribution of L1 in the adult mouse brain by immunohistochemistry using two polyclonal antibodies against different epitopes for L1. Immunoreactive L1 was widely but unevenly distributed from the olfactory bulb to the upper cervical cord. The accumulation of immunoreactive L1 was greatest in a non-neuronal element of the major fibre bundles, i.e. the lateral olfactory tract, olfactory and temporal limb of the anterior commissure, corpus callosum, stria terminalis, globus pallidus, fornix, mammillothalamic tract, solitary tract, and spinal tract of the trigeminal nerve. High to highest levels of non-neuronal and neuronal L1 were found in the grey matter; i.e. the piriform and entorhinal cortices, hypothalamus, reticular part of the substantia nigra, periaqueductal grey, trigeminal spinal nucleus etc. High to moderate density of neuronal L1 was found in the olfactory bulb, layer V of the cerebral cortex, amygdala, pontine grey, superior colliculi, cerebellar cortex, solitary tract nucleus etc. Only low to lowest levels of neuronal L1 were found in the hippocampus, grey matter in the caudate-putamen, thalamus, cerebellar nuclei etc. L1 is widely and unevenly distributed in the matured mouse brain, where immunoreactivity was present not only in neuronal elements; axons, synapses and cell soma, but also in non-neuronal elements.
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影响因子：
2.5
作者：
MILLER, PD;CHUNG, WW;DEKOSKY, ST
通讯作者：
DEKOSKY, ST
影响因子：
16.2
作者：
IGNELZI, MA;MILLER, DR;MANESS, PF
通讯作者：
MANESS, PF
影响因子：
3.3
作者：
Sandi, C;Merino, JJ;Venero, C
通讯作者：
Venero, C
DOI：
10.1002/neu.1071
发表时间：
2001-11-05
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
Schuster，T;Krug，M;Schachner，M
通讯作者：
Schachner，M
影响因子：
16.2
作者：
LEMMON, V;FARR, KL;LAGENAUR, C
通讯作者：
LAGENAUR, C