An electrochemical glucose biosensor exploiting a polyaniline grafted multiwalled carbon nanotube/perfluorosulfonate ionomer-silica nanocomposite
An electrochemical glucose biosensor exploiting a polyaniline grafted multiwalled carbon nanotube/perfluorosulfonate ionomer-silica nanocomposite
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DOI：
10.1016/j.biomaterials.2009.07.047
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发表时间：
2009-10-01
期刊：
影响因子：
14
通讯作者：
Lee, Jong W.
中科院分区：
文献类型：
--
作者：
Gopalan, Anantha I.;Lee, Kwang P.;Lee, Jong W.
将葡萄糖氧化酶(GOx)负载到有机-无机杂化纳米复合材料中，制备了葡萄糖生物传感器。制备过程包括将二氧化硅网络形成Nafion(全氟磺酸离聚体)，然后将聚苯胺接枝多壁碳纳米管(MWNT-g-PANI)负载到Nafion-SiO_2纳米复合材料上。场发射扫描电子显微镜(FE-SEM)显示，Nafion-SiO_2/MWNT-g-PANI复合材料中存在球形的二氧化硅颗粒(尺寸在250 nm-1微米)和管状的MWNT-g-PANI颗粒。采用计时电流法和循环伏安法考察了该传感器对葡萄糖的检测性能。Nafion-SiO_2/MWCNT-g-PANI/GOX生物传感器对葡萄糖的线性响应范围为110mM，相关系数为0.9972，灵敏度为5.01mA/mm，响应时间较短(与6 S相近)，重复性好(相对标准偏差为2.2%)，长期稳定性好。Nafion和MWNT-g-PANI中二氧化硅网络的存在协同作用有助于生物传感器对葡萄糖的电化学检测。(C)2009爱思唯尔有限公司。保留所有权利。
A glucose biosensor was fabricated with loading of glucose oxidase (GOx) into a new organic-inorganic hybrid nanocomposite. The preparation involves formation of silica network into a Nafion (per-fluorosulfonate ionomer) and subsequent loading of polyaniline grafted multiwalled carbon nanotubes (MWNT-g-PANI) onto Nafion-silica nanocomposite. Field emission scanning electron microscopy (FE-SEM) of Nafion-silica/MWNT-g-PANI composite reveals the presence of spherical silica particles (sizes in the range 250 nm-1 mu m) and tubular MWNT-g-PANI particles. Chronoamperometry and cyclic voltammetry were used to evaluate the performance of biosensor towards glucose. The Nafion-silica/MWCNT-g-PANI/GOx biosensor exhibited a linear response to glucose in the concentration range of 110 mm with a correlation coefficient of 0.9972, good sensitivity (5.01 mu A/mM), a low response time (similar to 6 s), repeatability (R.S.D value of 2.2%) and along-term stability. The presence of silica network within Nafion and MWNT-g-PANI synergistically contributes to the performance of the biosensor towards the electrochemical detection of glucose. (C) 2009 Elsevier Ltd. All rights reserved.