Cytokines differentially regulate ICAM-1 and VCAM-1 expression on human gingival fibroblasts
Cytokines differentially regulate ICAM-1 and VCAM-1 expression on human gingival fibroblasts
复制标题
DOI：
10.1111/j.1365-2249.2006.03064.x
复制
发表时间：
2006-06-01
影响因子：
4.6
通讯作者：
Matsuo, T
中科院分区：
文献类型：
--
作者：
Hosokawa, Y;Hosokawa, I;Matsuo, T
人牙龈成纤维细胞（HGF）表达细胞间粘附分子-1（ICAM-1）和血管粘附分子-1（VCAM-1）可能是牙周病组织炎症细胞迁移和滞留的重要机制。本研究旨在评估哪些细胞因子调节HGF上ICAM-1和VCAM-1的表达。肿瘤坏死因子（TNF）-α和干扰素（IFN）-γ增强HGF上ICAM-1和VCAM-1的表达。IL-1 β主要上调ICAM-1表达。另一方面，IL-4和IL-13仅增强HGF上VCAM-1的表达。IL-10不调节ICAM-1和VCAM-1的表达。转化生长因子（TGF）-β 1促进ICAM-1表达。然而，TGF-β 1抑制TNF-α或IL-4诱导的VCAM-1表达。核因子-κ B（NF-κ B）激活抑制剂MG-132可抑制HGF诱导的ICAM-1和VCAM-1表达。丝裂原活化蛋白激酶（MAPK）抑制剂不影响TNF-α诱导的ICAM-1表达。有趣的是，VCAM-1表达被MEK抑制剂（PD 98059）和c-Jun NH 2-末端激酶（JNK）抑制剂（SP 600125）增强。提示牙周病组织中细胞因子的平衡可能是调控HGF表面ICAM-1和VCAM-1表达的重要因素，而ICAM-1和VCAM-1表达的平衡可能是调节牙周病组织中白细胞浸润和滞留的重要因素。
The expression of intercellular adhesion molecule-1 (ICAM-1) and vascular adhesion molecule-1 (VCAM-1) on human gingival fibroblasts (HGF) may be important for migration and retention of inflammatory cells in periodontally diseased tissue. This study aimed to assess which cytokines regulate ICAM-1 and VCAM-1 expression on HGF. Tumour necrosis factor (TNF)-alpha and interferon (IFN)-gamma enhanced both ICAM-1 and VCAM-1 expression on HGF. Interleukin (IL)-1 beta mainly up-regulated ICAM-1 expression. On the other hand, IL-4 and IL-13 enhanced only VCAM-1 expression on HGF. IL-10 did not modulate both ICAM-1 and VCAM-1 expression. Transforming growth factor (TGF)-beta 1 enhanced ICAM-1 expression. However, TGF-beta 1 inhibited the VCAM-1 expression induced by TNF-alpha or IL-4. Both ICAM-1 and VCAM-1 expression by HGF was inhibited by nuclear factor-kappaB (NF-kappa B) activation inhibitor (MG-132). Mitogen-activated protein kinases (MAPK) inhibitors did not influence ICAM-1 expression induced by TNF-alpha. Interestingly, VCAM-1 expression was enhanced by MEK inhibitor (PD98059) and c-Jun NH2-terminal kinase (JNK) inhibitor (SP600125). These results mean that the balance of cytokines in periodontally diseased tissue may be essential for control of ICAM-1 and VCAM-1 expression on HGF, and the balance of ICAM-1 and VCAM-1 expression might be important for regulation of leucocytes infiltration and retention in periodontally diseased tissue.