Calcineurin B-Like Proteins CBL4 and CBL10 Mediate Two Independent Salt Tolerance Pathways in Arabidopsis
Calcineurin B-Like Proteins CBL4 and CBL10 Mediate Two Independent Salt Tolerance Pathways in Arabidopsis
复制标题
DOI：
10.3390/ijms20102421
复制
发表时间：
2019-05
影响因子：
5.6
通讯作者：
Yang Yang-Yang;Chi Zhang;R. Tang;Hai-xia Xu;Wenzhi Lan;Fugeng Zhao;S. Luan
中科院分区：
文献类型：
--
作者：
Yang Yang-Yang;Chi Zhang;R. Tang;Hai-xia Xu;Wenzhi Lan;Fugeng Zhao;S. Luan
在拟南芥中，盐过度敏感 (SOS) 途径由钙调神经磷酸酶 B 样蛋白 4 (CBL4/SOS3)、CBL 相互作用蛋白激酶 24 (CIPK24/SOS2) 和 SOS1 组成，已被明确定义为通过将 Na+ 挤出到细胞外空间来控制细胞离子稳态的关键机制，从而赋予植物耐盐性。 CBL10 还在耐盐性中发挥着关键作用，这可能是通过激活液泡中的 Na+ 区室而实现的。然而，SOS 和 CBL10 调节过程的功能关系仍不清楚。在这里，我们分析了 CBL4 和 CBL10 之间的遗传相互作用，发现与 cbl4 和 cbl10 单突变体相比，cbl4 cbl10 双突变体对盐更加敏感，表明 CBL4 和 CBL10 各自指导不同的耐盐途径。此外，cbl4 cbl10 和 cipk24 cbl10 双突变体比 cipk24 单突变体更敏感，表明 CBL10 指导涉及 CIPK24 和不同于 SOS 途径的其他伙伴的过程。尽管cbl4 cbl10、cipk24 cbl10和sos1 cbl10双突变体在整个植物水平上表现出与sos1相当的盐敏感表型，但它们在高盐条件下积累的Na+都比sos1低得多，这表明CBL10调节额外的未知转运过程，这些过程在Na+稳态中发挥与SOS1不同的作用。
In Arabidopsis, the salt overly sensitive (SOS) pathway, consisting of calcineurin B-like protein 4 (CBL4/SOS3), CBL-interacting protein kinase 24 (CIPK24/SOS2) and SOS1, has been well defined as a crucial mechanism to control cellular ion homoeostasis by extruding Na+ to the extracellular space, thus conferring salt tolerance in plants. CBL10 also plays a critical role in salt tolerance possibly by the activation of Na+ compartmentation into the vacuole. However, the functional relationship of the SOS and CBL10-regulated processes remains unclear. Here, we analyzed the genetic interaction between CBL4 and CBL10 and found that the cbl4 cbl10 double mutant was dramatically more sensitive to salt as compared to the cbl4 and cbl10 single mutants, suggesting that CBL4 and CBL10 each directs a different salt-tolerance pathway. Furthermore, the cbl4 cbl10 and cipk24 cbl10 double mutants were more sensitive than the cipk24 single mutant, suggesting that CBL10 directs a process involving CIPK24 and other partners different from the SOS pathway. Although the cbl4 cbl10, cipk24 cbl10, and sos1 cbl10 double mutants showed comparable salt-sensitive phenotype to sos1 at the whole plant level, they all accumulated much lower Na+ as compared to sos1 under high salt conditions, suggesting that CBL10 regulates additional unknown transport processes that play distinct roles from the SOS1 in Na+ homeostasis.