Peripheral circadian rhythms in the liver and white adipose tissue of mice are attenuated by constant light and restored by time-restricted feeding
Peripheral circadian rhythms in the liver and white adipose tissue of mice are attenuated by constant light and restored by time-restricted feeding
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DOI：
10.1371/journal.pone.0234439
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发表时间：
2020-06-12
期刊：
影响因子：
3.7
通讯作者：
Ishibashi, Shun
中科院分区：
文献类型：
--
作者：
Yamamuro, Daisuke;Takahashi, Manabu;Ishibashi, Shun
昼夜节律紊乱是多种代谢性疾病的基础。已知持续光照（LL）会扰乱中枢和外周昼夜节律。在此，我们试图确定LL的影响在不同外周组织之间是否存在差异，以及限时进食是否能恢复昼夜节律，特别是在白色脂肪组织（WAT）中。将6周龄的小鼠置于三种进食模式下：在光/暗周期（LD）下自由进食，在LL周期下自由进食，以及在LL周期下夜间限时进食且给予正常食物。3周后，我们比较了体重、食物摄入量、血脂和血糖水平，以及肝脏和WAT中时钟基因和参与脂质代谢的基因的表达模式。在有或没有限时进食的LL条件下饲养的小鼠体重重5.2%（p<0.05），但食物摄入量无差异。LL导致肝脏和WAT中时钟基因和脂质代谢相关基因的表达紊乱。限时进食部分恢复了WAT中时钟基因和脂质代谢相关基因的表达，但对肝脏中的这些基因表达无影响。LL还导致血浆甘油三酯和血糖水平升高，限时进食可逆转这些变化。这些结果表明，LL对肝脏和WAT中的昼夜节律和代谢有不同影响，限时进食可恢复WAT中的昼夜节律和代谢功能。
Disturbance of circadian rhythms underlies various metabolic diseases. Constant light exposure (LL) is known to disrupt both central and peripheral circadian rhythms. Here, we attempted to determine whether the effects of LL are different between various peripheral tissues and whether time-restricted feeding restores the circadian rhythms especially in white adipose tissue (WAT). Six-week-old mice were subjected to three feeding regimes:ad libitumfeeding under light/dark phase (LD),ad libitumfeeding under LL cycle, and restricted feeding at night-time under LL cycle with a normal chow. After 3 weeks, we compared body weight, food intake, plasma levels of lipids and glucose, and the expression patterns of the clock genes and the genes involved in lipid metabolism in the liver and WAT. The mice kept under LL with or without time-restricted feeding were 5.2% heavier (p