MicroRNA-410-5p as a potential serum biomarker for the diagnosis of prostate cancer.
MicroRNA-410-5p as a potential serum biomarker for the diagnosis of prostate cancer.
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MicroRNA-410-5p 作为诊断前列腺癌的潜在血清生物标志物
DOI：
10.1186/s12935-016-0285-6
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发表时间：
2016
影响因子：
5.8
通讯作者：
Liu S
中科院分区：
文献类型：
--
作者：
Wang J;Ye H;Zhang D;Hu Y;Yu X;Wang L;Zuo C;Yu Y;Xu G;Liu S
前列腺癌（PCa）由于其多变的表现和缺乏可靠的诊断工具，仍然是一个诊断挑战。MicroRNAs （miRNAs）在广泛的病理生理过程中调控基因。血浆mirna是心衰、糖尿病和其他疾病的理想生物标志物。然而，使用循环mirna作为诊断PCa的生物标志物仍然未知。方法149例前列腺癌患者，57例健康对照，121例非癌性前列腺增生及其他泌尿系统疾病患者。采用miRNA逆转录和基于sybr - green的双标准曲线miRNA定量聚合酶链反应（qPCR）评估miR-410-5p的异常表达。采用受试者操作特征（ROC）曲线分析评估miR-410-5p作为替代生物标志物的诊断准确性。结果前列腺癌患者循环miRNA-410-5p （miR-410-5p）水平明显高于健康对照组和非癌症患者。ROC曲线分析显示，血浆miR-410-5p是PCa的特异性诊断生物标志物，曲线下面积（AUC）为0.8097（95%置信区间，0.7371-0.8823;P < 0.001）。结论血清miR-410-5p水平是诊断前列腺癌的潜在生物标志物。
BackgroundProstate cancer (PCa) remains to be a diagnostic challenge due to its variable presentation and the lack of reliable diagnosis tool. MicroRNAs (miRNAs) regulate gene in extensive range of pathophysiologic processes. Plasma miRNAs are ideal biomarkers in heart failure, diabetes and other disease. However, using circulating miRNAs as biomarkers for the diagnosis of PCa is still unknown.Methods149 PCa patients, 57 healthy controls, and 121 non-cancer patients (benign prostatic hyperplasia and other urinary diseases) were enrolled in this study. The reverse transcription of miRNA and SYBR-Green-based double standards curve miRNA quantitative polymerase chain reactions (qPCR) were used to evaluate the dysregulated miR-410-5p. Receiver operator characteristic (ROC) curve analysis was used to evaluate the diagnostic accuracy of miR-410-5p identified as the alternative biomarker.ResultsCirculating miRNA-410-5p (miR-410-5p) level was significantly higher in the PCa patients than in healthy controls or non-cancer patients. ROC curve analysis showed that plasma miR-410-5p was a specific diagnostic biomarker of PCa with an area under curve(AUC) of 0.8097 (95 % confidence interval, 0.7371–0.8823; P < 0.001).ConclusionsThe serum miR-410-5p level is a potential biomarker for the diagnosis of PCa.