The density of extracellular matrix proteins regulates inflammation and insulin signaling in adipocytes
The density of extracellular matrix proteins regulates inflammation and insulin signaling in adipocytes
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DOI：
10.1016/j.febslet.2010.08.033
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发表时间：
2010-10-08
期刊：
影响因子：
3.5
通讯作者：
Funaki, Makoto
中科院分区：
文献类型：
--
作者：
Li, Qinkai;Hata, Akiko;Funaki, Makoto
细胞不仅可以感知微环境中存在的细胞外基质（ECM）蛋白的类型，还可以感知其密度。在这里，我们研究了 ECM 蛋白密度对脂肪细胞中脂肪因子分泌和胰岛素信号传导的影响。为此，将 3T3-L1 脂肪细胞培养在涂有梯度密度的 I 型胶原蛋白和纤连蛋白混合物的聚丙烯酰胺凝胶表面上。我们发现高密度 ECM 会导致胰岛素信号传导和脂联素分泌减少，而单核细胞趋化蛋白 1 (MCP-1) 的分泌则通过核因子 kappa B (NF-kappa B) 的激活而增加。这些结果表明 ECM 的密度直接调节脂肪细胞的炎症反应和胰岛素敏感性。 结构摘要：MINT-7992217：Irs1 (uniprotkb:P35569) 与磷脂酰肌醇 3-激酶 85 kDa 调节亚基 α 发生物理相互作用 (MI:0915) (uniprotkb:P26450) 通过抗诱饵免疫共沉淀 (MI: 0006) (C) 2010 年欧洲生化协会联合会。由 Elsevier B.V 出版。保留所有权利。
Cells can not only sense the type of extracellular matrix (ECM) protein that is present in the microenvironment, but they can also sense its density. Here, we investigated the effects of ECM protein density on adipokine secretion and insulin signaling in adipocytes. To this end, 3T3-L1 adipocytes were cultured on the surface of polyacrylamide gels that were coated with gradient densities of a collagen type I and fibronectin mixture. We found that high density ECM causes a decrease in insulin signaling and adiponectin secretion, whereas the secretion of monocyte chemoattractant protein-1 (MCP-1) was increased via the activation of nuclear factor-kappa B (NF-kappa B). These results indicate that the density of the ECM directly regulates the inflammatory response and insulin sensitivity of adipocytes.Structured summary:MINT-7992217: Irs1 (uniprotkb:P35569) physically interacts (MI:0915) with phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha (uniprotkb:P26450) by anti bait coimmunoprecipitation (MI: 0006) (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.