THE EFFECT OF HYPERTHERMIA ON HUMAN PROSTATIC-CARCINOMA CELL-LINES - EVALUATION INVITRO
THE EFFECT OF HYPERTHERMIA ON HUMAN PROSTATIC-CARCINOMA CELL-LINES - EVALUATION INVITRO
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DOI：
10.1016/s0022-5347(17)41090-1
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发表时间：
1989-04-01
影响因子：
6.6
通讯作者：
KOONTZ, WW
中科院分区：
文献类型：
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作者：
KAVER, I;WARE, JL;KOONTZ, WW
体外研究了热疗对已建立的人前列腺癌细胞系（PC-3、DU-145）和相关亚系（1-LN、125-1L）的影响。将细胞在43℃或37℃下进行不同时间间隔的热处理（一小时或两小时），并通过集落形成测定和通过测量细胞生长速率来评估细胞存活率。虽然在 43°C 下暴露一小时确实显示出对集落形成的平均抑制，范围为 29% 至 41%，但在暴露两小时时观察到抑制率显着增加（p < 0.001），范围为 57% 至 92%。本研究报告了热疗对已建立的人类前列腺肿瘤细胞系的细胞毒性作用。这些体外结果表明热疗可能成为局部控制前列腺癌的一种潜在有用的辅助治疗形式。然而，当这种新的癌症治疗方式被提议进行临床试验时，温度和暴露时间可能会对细胞杀死产生重要影响。
The effect of hyperthermia on established human prostate carcinoma cell lines (PC-3, DU-145) and related sublines (1-LN, 125-1L) was investigated in vitro. Cells were exposed to heat treatment at 43C or 37C for varying time intervals, (one hr or two hrs) and cell survival was evaluated by the colony formation assay and by measurement of cellular growth rate. While one hr exposure at 43C did show a mean inhibition of colony formation, ranging from 29 to 41%, a statistically significant increase in inhibition rate (p < 0.001) was observed at two hr exposure, ranging from 57 to 92%. This study is a report of the cytotoxic effect of hyperthermia on established human prostatic tumor cell lines. These in vitro results indicate that hyperthermia may become a potentially useful form of adjunctive therapy for local control of prostatic cancer. However, the temperature and exposure time may have an important impact on cell kill when this new modality for cancer treatment is proposed for a clinical trial.