EVIDENCE FOR GENERATION OF AN ELECTRONIC EXCITATIONS STATES) IN HUMAN POLYMORPHONUCLEAR LEUKOCYTES AND ITS PARTICIPATION IN BACTERICIDAL ACTIVITY
EVIDENCE FOR GENERATION OF AN ELECTRONIC EXCITATIONS STATES) IN HUMAN POLYMORPHONUCLEAR LEUKOCYTES AND ITS PARTICIPATION IN BACTERICIDAL ACTIVITY
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DOI：
10.1016/0006-291x(72)90545-1
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发表时间：
1972-01-01
影响因子：
3.1
通讯作者：
STJERNHOLM, RL
中科院分区：
文献类型：
--
作者：
ALLEN, RC;STEELE, RH;STJERNHOLM, RL
热灭活调理丙酸杆菌，shermanii，（P.，shermanii）和调理的活金黄色葡萄球菌（S.，金黄色葡萄球菌）在吞噬作用下引起人多形核白细胞（PMN）的化学发光（CL），但不引起淋巴细胞的化学发光。CL与磷酸己糖（HMP）分流活性、PMN数量和细菌数量相关。中性粒细胞经乳胶颗粒调理后诱发CL。没有CL从PMN暴露于血清单独，也没有unopsonized细菌。有人提出，中性粒细胞的CL反映了单线态氧1 0 2的产生，单线态氧本身充当杀菌和/或CL物质。
Heat-killed opsonized Propionibacterium, shermanii,(P., shermanii) and opsonized live Staphylococcus, aureus,(S., aureus) are shown to elicit a chemiluminescence (CL) from human polymorphonuclear leukocytes (PMN) but not from lymphocytes upon phagocytosis. The CL is correlated with hexose monophosphate (HMP) shunt activity, number of PMN, and bacteria. CL was evoked from PMN by opsonized latex particles. No CL was obtained from PMN exposed to serum alone, nor unopsonized bacteria. It is proposed that the CL of PMN reflects the generation of singlet oxygen, 1 0 2, which acts per, se, as the bactericidal and/or CL species.