Factors contributing to the development of chronic rejection in heterotopic rat heart transplantation
Factors contributing to the development of chronic rejection in heterotopic rat heart transplantation
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DOI：
10.1097/00007890-199707270-00007
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发表时间：
1997-07-27
期刊：
影响因子：
6.2
通讯作者：
Tilney, NL
中科院分区：
文献类型：
--
作者：
Schmid, C;Heemann, U;Tilney, NL
背景本研究旨在阐明免疫原性、免疫抑制和缺血对移植物血管病变（TVP）发生的影响，以及研究同基因移植中的肌内膜增殖。Fischer 344和Brown Norway大鼠同种异体心脏移植物和刘易斯同种异体心脏移植物用雷帕霉素或环孢素处理，暴露于4小时的冷缺血，观察100至300天，然后评估TVP和血管周围浸润的发生率和程度。Fischer 344-->刘易斯同种异体移植物（雷帕霉素，0.5 mg/kg，持续14天）中TVP的发生率稳步上升，在所有时间，冠状动脉病变中均存在密集的单核细胞浸润（从50天的10+/-2%到150天的85+/-15%）。免疫原性增加（Brown Norway-->刘易斯）强化了TVP（62+/-13%），与对照组（25+/-15%，P
Background. The present study was devised to elucidate the influence of immunogenicity, immunosuppression, and ischemia on the development of transplant vasculopathy (TVP) as well as to investigate myointimal proliferation in syngeneic transplantation.Methods. Fischer 344 and Brown Norway rat heart allografts and Lewis isografts were treated with rapamycin or cyclosporine, exposed to 4 hr of cold ischemia, and observed for 100 to 300 days before the incidence and degree of TVP and perivascular infiltration were assessed.Results. The incidence of TVP in Fischer 344-->Lewis allografts (rapamycin, 0.5 mg/kg for 14 days) rose steadily, with dense mononuclear infiltration present in coronary lesions at all times (from 10+/-2% at 50 days to 85+/-15% at 150 days). Increased immunogenicity (Brown Norway-->Lewis) intensified TVP (62+/-13%) as compared with its control (25+/-15%, P