Micelle to trapping solution stacking in micellar electrokinetic chromatography.
Micelle to trapping solution stacking in micellar electrokinetic chromatography.
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DOI：
10.1016/j.chroma.2009.11.038
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
Lihong Liu;Xinxia Deng;Xingguo Chen
中科院分区：
文献类型：
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作者：
Lihong Liu;Xinxia Deng;Xingguo Chen
建立了胶束电动色谱(MEKC)在酸性缓冲液中捕获溶液堆积(MSS)的分析策略。堆积机理是基于与胶束载体结合的分子的传输、释放和捕获，这些分子被折叠成捕集溶液(TS)，作为容纳和堆积分析物的介质。以十二烷基硫酸钠(十二烷基硫酸钠)胶束为载体，以汉防己甲素和防己内酯为模型混合物，验证了这种堆积方法。进行了MSS-MEKC实验，并与普通MEKC实验结果进行了比较。结果表明，采用含8.0mM十二烷基硫酸钠和含50mMH3PO4-55%(v/v)乙醇的样品基质，可使分析物的检测灵敏度提高113~123倍，并在最佳条件下完成了粉防己碱和防风痛安胶囊中粉防己碱和防己内酯的分离和测定。
An analytical strategy micelle to trapping solution stacking (MSS) was developed in acidic buffer in micellar electrokinetic chromatography (MEKC). The stacking mechanism is based on the transport, release, capturing of molecules bound to micelle carriers that are made to collapse into trapping solution (TS) to serve as the medium to contain and stacking the analytes. Tetrandrine and fangchinoline were selected as model mixture using sodium dodecyl sulfate (SDS) micelles as carrier to demonstrate this stacking method. The experiments by MSS-MEKC were carried out and further compared with those by normal MEKC. The results reveal that 113–123-fold improvements in the detection sensitivity was obtained for the analytes, and separation and determination of tetrandrine and fangchinoline in Stephaniae tetrandrae S. Moore and Fengtongan capsules were finished under optimum conditions using the sample matrix containing 8.0mM SDS and TS containing 50mM H3PO4–55% (v/v) ethanol.