The RNA binding domain of jerky consists of tandemly arranged helix-turn-helix/homeodomain-like motifs and binds specific sets of mRNAs
The RNA binding domain of jerky consists of tandemly arranged helix-turn-helix/homeodomain-like motifs and binds specific sets of mRNAs
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DOI：
10.1128/mcb.23.12.4083-4093.2003
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发表时间：
2003-06-01
影响因子：
5.3
通讯作者：
Toth, M
中科院分区：
文献类型：
--
作者：
Liu, WC;Seto, J;Toth, M
缺乏Jerky蛋白的小鼠会导致反复发作，让人联想到颞叶癫痫。Jerky存在于神经元中的mRNA颗粒中。我们发现Jerky的N-末端168个氨基酸对于mRNA结合是必要的和足够的。结合结构域类似于着丝粒结合蛋白B的两个串联排列的同源结构域样螺旋-转角-螺旋DNA结合基序。Jerky的螺旋-转角-螺旋基序也可以结合双链DNA，代表了一种新的哺乳动物RNA/DNA结合结构域。微阵列分析鉴定了编码参与核糖体组装和细胞应激反应的蛋白质的mRNA，所述蛋白质在体外和体内特异性地结合到Jerky的RNA结合结构域。这些数据表明Jerky缺陷小鼠的癫痫发生最有可能涉及与核糖体生物合成和神经元存活和/或凋亡相关的途径。
A deficit in the Jerky protein in mice causes recurrent seizures reminiscent of temporal lobe epilepsy. Jerky is present in mRNA particles in neurons. We show that the N-terminal 168 amino acids of Jerky are necessary and sufficient for mRNA binding. The binding domain is similar to the two tandemly arranged homeodomain-like helix-turn-helix DNA binding motifs of centromere binding protein B. The putative helix-turn-helix motifs of Jerky can also bind double-stranded DNA and represent a novel mammalian RNA/DNA binding domain. Microarray analysis identified mRNAs encoding proteins involved in ribosome assembly and cellular stress response that specifically bound to the RNA binding domain of Jerky both in vitro and in vivo. These data suggest that epileptogenesis in Jerky-deficient mice most likely involves pathways associated with ribosome biogenesis and neuronal survival and/or apoptosis.