Reactive oxygen species and DNA damage after ultrasound exposure
Reactive oxygen species and DNA damage after ultrasound exposure
复制标题
DOI：
10.1016/j.bioeng.2007.02.001
复制
发表时间：
2007-06-01
期刊：
影响因子：
--
通讯作者：
Gabryelak, Teresa
中科院分区：
文献类型：
--
作者：
Milowska, Katarzyna;Gabryelak, Teresa
这项工作的目的是检测超声(US)照射后过氧化氢和羟基自由基的形成，并验证超声诱导的活性氧物种可导致DNA损伤的假设。1 MHz连续超声以0.61~2.44W/cm(2)的声强作用于孵育液中，可观察到细胞内有氧自由基的形成。超声波照射细胞产生的自由基和过氧化氢会导致DNA损伤。用彗星实验检测超声对DNA损伤程度的影响。用相同强度和频率的超声波对鱼类有核红细胞进行体外辐照。人们注意到，在所有使用的强度下，超声波都会引起DNA损伤。过氧化氢酶的加入并不能消除过氧化氢对DNA的损伤作用，说明过氧化氢引起的DNA损伤并不完全是过氧化氢引起的。结果表明，DNA损伤可以得到修复，这种修复机制在超声作用30min和60min后效果最好。此外，我们还研究了超声波增敏剂(锌和氯化铝)对DNA的损伤作用。人们注意到，单独或在超声波作用下，酞菁化合物(PC)对DNA损伤水平没有明显的影响。(C)2007爱思唯尔B.V保留所有权利。
The aim of this work was to detect the formation of hydrogen peroxide and hydroxyl radicals after ultrasound (US) exposure and test the hypothesis that reactive oxygen species induced by ultrasound can contribute to DNA damage. Formation of reactive oxygen species was observed in incubated medium after sonication with 1 MHz continuous ultrasound at the intensities of 0.61-2.44 W/cm(2). Free radicals and hydrogen peroxide produced by ultrasound exposure of cells can lead to DNA damage. Comet assay was used to assess the effect of ultrasound on the level of nuclear DNA damage. The nucleated erythrocytes from fish were exposed in vitro to ultrasound at the same intensities and frequency. It was noticed that ultrasound in all used intensities induced DNA damage. The effect was not eliminated by the addition of catalase, which indicates that DNA damage was not caused by hydrogen peroxide only. The results showed that the DNA damage can be repair and this mechanism was the most effective after 30 and 60 min after sonication. Furthermore, the ultrasound-induced DNA damage in the presence of sonosensitizer (Zn- and AICI-phthalocyanine) was studied. It was noticed that phthalocyaniens (Pcs) alone or with ultrasound did not induce significant changes in the level of DNA damage. (c) 2007 Elsevier B.V All rights reserved.