Enhanced infectivity of strains of Helicobacter pylori isolated from children compared with parental strains
Enhanced infectivity of strains of Helicobacter pylori isolated from children compared with parental strains
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与亲代菌株相比，从儿童体内分离出的幽门螺杆菌菌株的传染性增强
DOI：
10.1099/jmm.0.000918
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发表时间：
2019
影响因子：
3
通讯作者：
Kamiya Shigeru
中科院分区：
文献类型：
--
作者：
Zaman Cynthia;Osaki Takako;Furuta Yoshikazu;Hojo Fuhito;Yonezawa Hideo;Konno Mutsuko;Kurata Satoshi;Hanawa Tomoko;Kamiya Shigeru
目的：在日本等发达国家，家庭内感染，即母婴感染，被认为是幽门螺杆菌的主要传播途径之一。家庭内传播在h的致病性中起主要作用。幽门螺杆菌现在是没有争议的，尽管主要的传播途径仍然鲜为人知。我们进行这项研究是为了阐明决定家庭内传播的因素。方法：我们使用了几种H。从家庭成员中分离到幽门螺杆菌K21和K22菌株，K25菌株从家里的第三个孩子中分离出来。对长爪沙土鼠接种H。在每次实验中比较三种菌株对幽门螺杆菌的感染能力。此外，还测定了三株猪瘟病毒的全基因组序列、与胃上皮细胞的粘附性以及在静态或连续流培养条件下的生长。结果/关键发现：在所有四组动物中，大多数菌落被确定为相同的分子类型K25。优势菌株为幽门螺杆菌K25。体外分析表明，K25菌株的黏附能力强于其他两株菌株。通过评估h.3株幽门螺杆菌的TnPZ区仅在K25菌株中检出。幽门螺杆菌分离株、家庭内感染和动物感染由每个菌株的黏附能力和分子类型决定。
Purpose.Intra-familial infection, mother-to-child infection, is considered to be one of the main routes of transmission forHelicobacter pylori ,in developed countries such as Japan. A major role for intra-familial spread in the pathogenicity ofH. pyloriis now beyond controversy, although the major route of transmission remains poorly understood. We performed this study to clarify the factors determining intra-familial transmission.Methodology.We used severalH. pyloristrains isolated from family members to compare infectivity.H. pyloriK21 and K22 strains were isolated from the father and mother, and the K25 strain was isolated from the third child of the family. Mongolian gerbils were inoculated withH. pyloristrains and the infectivity of three strains was compared in each experiment. In addition, the whole genome sequence, adhesion to gastric epithelial cells and the growth of static condition or continuous flow culture among three strains ofH. pyloriwere analysed.Results/Key findings.Most of the colonies were determined as the same molecular type K25 in all of the four grouped animals andH. pyloriK25 was observed as the dominant strain. The stronger adhesion capacity of the K25 strain was observed in comparison with the other two strains throughin vitroanalysis. By assessing the genomic profiles ofH. pyloriisolates from three strains, identified TnPZ regions were detected only in the K25 strain.Conclusion.The infectivity ofH. pyloriisolates intra-familial infection and animal infection were prescribed by the adhesion capacity and molecular type of each strain.