Transgenic rice plants expressing a trypsin inhibitor are resistant against rice stem borers, Chilo suppressalis
Transgenic rice plants expressing a trypsin inhibitor are resistant against rice stem borers, Chilo suppressalis
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表达胰蛋白酶抑制剂的转基因水稻植物对二化螟具有抗性
DOI：
10.1046/j.1570-7458.1999.00576.x
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发表时间：
1999
影响因子：
1.9
通讯作者：
Y. Ohashi
中科院分区：
文献类型：
--
作者：
A. Mochizuki;Yaeko Nishizawa;H. Onodera;Y. Tabei;S. Toki;Y. Habu;M. Ugaki;Y. Ohashi
一种合成基因mwti 1b，编码一种四棱豆胰蛋白酶抑制剂WTI-1B，已被引入水稻植物中并表达。转胰蛋白酶抑制剂基因水稻植株的蛋白提取物在体外对水稻二化螟幼虫的肠道蛋白酶有抑制作用。与非转基因对照植物相比，在表达超过1 ng/10 μg总蛋白的WTI-1B的转基因水稻植物上饲养的幼虫的生长显著延迟。
A synthetic gene, mwti1b, coding for a winged bean trypsin inhibitor WTI‐1B, has been introduced and expressed in rice plants, Oryza sativa. Protein extracts from transgenic rice plants expressing the trypsin inhibitor inhibited the gut proteases of larvae of the serious insect pest, the rice stem borer, Chilo suppressalis (Lepidoptera: Pyralidae) in vitro. The growth of larvae reared on transgenic rice plants expressing WTI‐1B at more than 1 ng/10 μg total protein was significantly retarded compared to that on non‐transgenic control plants.