Quantitative disease resistance to the bacterial pathogen Xanthomonas campestris involves an Arabidopsis immune receptor pair and a gene of unknown function
Quantitative disease resistance to the bacterial pathogen Xanthomonas campestris involves an Arabidopsis immune receptor pair and a gene of unknown function
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DOI：
10.1111/mpp.12298
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发表时间：
2016-05-01
影响因子：
4.9
通讯作者：
Roby, Dominique
中科院分区：
文献类型：
--
作者：
Debieu, Marilyne;Huard-Chauveau, Carine;Roby, Dominique
虽然定量抗病（QDR）是植物中一种持久和广谱的抗性形式，但对QDR基础基因的鉴定仍处于起步阶段。最近有报道称，RKS1 （Resistance relatedKinaSe1）可使拟南芥中的大多数（但不是所有）细菌病原菌黄单胞菌（Xanthomonas campestris pv）产生QDR。定(Xcc)。因此，我们探索了拟南芥在不同种间的QDR遗传基础。采用嵌套全基因组关联定位方法，将QDR相关的基因组区域精细定位到Xcc12824（2种人种）和XccCFBP6943（6种人种）。为了鉴定与QDR相关的基因，选择候选基因的插入突变体（T-DNA）并对Xc进行表型分析。我们确定了两个主要的qtl特异性地赋予Xcc12824和XccCFBP6943抗性。虽然Xcc12824的QDR是由编码未知功能蛋白的At5g22540赋予的，但XccCFBP6943的QDR涉及众所周知的免疫受体对RRS1/RPS4。除了RKS1外，本研究还揭示了三个基因参与了Xc的抗性，它们的特异性范围截然不同，这表明Xc的QDR涉及一个复杂的网络，整合了由不同病原体分子决定因素触发的多种反应途径。
Although quantitative disease resistance (QDR) is a durable and broad-spectrum form of resistance in plants, the identification of the genes underlying QDR is still in its infancy. RKS1 (Resistance relatedKinaSe1) has been reported recently to confer QDR in Arabidopsis thaliana to most but not all races of the bacterial pathogen Xanthomonas campestris pv. campestris (Xcc). We therefore explored the genetic bases of QDR in A.thaliana to diverse races of X.campestris (Xc). A nested genome-wide association mapping approach was used to finely map the genomic regions associated with QDR to Xcc12824 (race 2) and XccCFBP6943 (race 6). To identify the gene(s) implicated in QDR, insertional mutants (T-DNA) were selected for the candidate genes and phenotyped in response to Xc. We identified two major QTLs that confer resistance specifically to Xcc12824 and XccCFBP6943. Although QDR to Xcc12824 is conferred by At5g22540 encoding for a protein of unknown function, QDR to XccCFBP6943 involves the well-known immune receptor pair RRS1/RPS4. In addition to RKS1, this study reveals that three genes are involved in resistance to Xc with strikingly different ranges of specificity, suggesting that QDR to Xc involves a complex network integrating multiple response pathways triggered by distinct pathogen molecular determinants.