A prime-boost vaccination strategy using naked DNA followed by recombinant porcine adenovirus protects pigs from classical swine fever
A prime-boost vaccination strategy using naked DNA followed by recombinant porcine adenovirus protects pigs from classical swine fever
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DOI：
10.1016/s0378-1135(00)00388-6
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发表时间：
2001-05-21
影响因子：
3.3
通讯作者：
Johnson, MA
中科院分区：
文献类型：
--
作者：
Hammond, JM;Jansen, ES;Johnson, MA
用表达猪瘟病毒（CSFV） gp55/E2基因的裸质粒DNA接种6周龄断奶仔猪和7日龄断奶仔猪。然后给两组猪注射表达gp55基因的重组猪腺病毒（rPAV-gp55）加强剂。猪瘟病毒攻毒后，100%的断奶仔猪和75%的断奶仔猪免于感染猪瘟病毒。给予单剂量rPAV-gp55的断奶仔猪也受到保护，但在攻毒后立即表现出轻微的体温升高。然而。在rPAV-gp55之前接种DNA的断奶动物在攻毒后体温没有波动，死后脾脏或淋巴结没有病理变化。此外，在rPAV疫苗接种组和在攻毒后只有一只猪的免疫接种组中没有再分离出CSFV，这表明两种疫苗接种方案都有可能减少或阻止病毒的脱落。挑战。(C) 2001 Elsevier Science B.V.版权所有
Weaned pigs (6-week-old) and 7-day-old pre-weaned piglets were vaccinated with naked plasmid DNA expressing the gp55/E2 gene from classical swine fever virus (CSFV). Both groups of pigs were then given a booster dose of recombinant porcine adenovirus expressing the gp55 gene (rPAV-gp55). Following challenge with CSFV: 100% of weaned pigs and 75% pre-weaned piglets were protected from disease. Weaned pigs given a single dose of rPAV-gp55 were also protected, but showed a slight increase in temperature immediately post-challenge. However. weaned animals given a DNA prime before rPAV-gp55 showed no fluctuation in body temperature following challenge and no pathology in spleen or lymph nodes upon post-mortem, In addition, no CSFV could be re-isolated from the rPAV vaccinated group and from only one pig in the prime-boost group following challenge, suggesting that both vaccination regimes have the potential to reduce or prevent virus shedding following experimental. challenge. (C) 2001 Elsevier Science B.V. All rights reserved.