Suppression or knockout of SaF/SaM overcomes the Sa-mediated hybrid male sterility in rice
Suppression or knockout of SaF/SaM overcomes the Sa-mediated hybrid male sterility in rice
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SaF/SaM 的抑制或敲除克服了 Sa 介导的水稻杂种雄性不育
DOI：
10.1111/jipb.12564
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发表时间：
2017-09-01
影响因子：
11.4
通讯作者：
Chen, Letian
中科院分区：
文献类型：
--
作者：
Xie, Yongyao;Niu, Baixiao;Chen, Letian
水稻籼粳亚种之间的杂交通常是不育的，这阻碍了亚种间杂交育种中杂种优势的利用。复杂基因座Sa包含两个相邻的基因SaF和SaM，它们相互作用导致粳稻-籼稻杂种中携带粳稻等位基因的花粉粒败育。在这里，我们表明，通过RNA干扰沉默SaF或SaM可以恢复具有杂合Sa的籼粳杂交种的雄性育性。我们进一步使用基于成簇规则间隔短回文重复序列 (CRISPR)/Cas9 的基因组编辑分别敲除籼稻品系的 SaF 和 SaM 等位基因，以创建杂交兼容品系。由此产生的人工中性等位基因不会影响花粉活力和其他农业性状，但确实打破了杂交种的繁殖障碍。我们发现一些水稻品系具有天然中性等位基因Sa-n，其与杂交种中典型的粳稻或籼稻Sa等位基因相容。我们的结果表明，SaF 和 SaM 是杂种雄性不育所必需的，但对于花粉发育来说并不是必需的。本研究通过敲除Sa基因座或利用天然Sa-n等位基因克服水稻育种中的杂种雄性不育性，为产生杂交相容系提供了有效的方法。 (c) 2017 年作者。生物电磁学由 Wiley periodicals, Inc. 出版
Hybrids between the indica and japonica subspecies of rice (Oryza sativa) are usually sterile, which hinders utilization of heterosis in the inter-subspecific hybrid breeding. The complex locus Sa comprises two adjacently located genes, SaF and SaM, which interact to cause abortion of pollen grains carrying the japonica allele in japonica-indica hybrids. Here we showed that silencing of SaF or SaM by RNA interference restored male fertility in indica-japonica hybrids with heterozygous Sa. We further used clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-based genome editing to knockout the SaF and SaM alleles, respectively, of an indica rice line to create hybrid-compatible lines. The resultant artificial neutral alleles did not affect pollen viability and other agricultural traits, but did break down the reproductive barrier in the hybrids. We found that some rice lines have natural neutral allele Sa-n, which was compatible with the typical japonica or indica Sa alleles in hybrids. Our results demonstrate that SaF and SaM are required for hybrid male sterility, but are not essential for pollen development. This study provides effective approaches for the generation of hybrid-compatible lines by knocking out the Sa locus or using the natural Sa-n allele to overcome hybrid male sterility in rice breeding. (c) 2017 The Authors. Bioelectromagnetics published by Wiley Periodicals, Inc.