Meiotic synapsis proceeds from a limited number of subtelomeric sites in the human male
Meiotic synapsis proceeds from a limited number of subtelomeric sites in the human male
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DOI：
10.1086/468188
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发表时间：
2005-10-01
影响因子：
9.8
通讯作者：
Hassold, TJ
中科院分区：
文献类型：
--
作者：
Brown, PW;Judis, L;Hassold, TJ
突触复合体（synaptonemal complex， SC）的形成是减数分裂过程中至关重要的早期步骤，但对人类SC的建立知之甚少。因此，我们最近开始了一项关于人类男性突触的研究，结合免疫荧光和荧光原位杂交方法分析了来自一系列对照个体的前期精母细胞。我们的研究结果表明，突触是一个受到严格调控的过程，个体之间的差异相对较小。在非顶心染色体上，有两个突触起始位点，一个在远端短臂上，一个在远端长臂上，而顶心染色体在远端长臂上只有一个位点。对于这两种类型的染色体，突触随后向着丝粒进行，几乎没有证据表明特定的p臂或q臂序列会影响这一过程。然而，着丝粒似乎对突触有抑制作用——也就是说，当一条非单中心染色体的一条臂在另一条臂之前被“拉上”时，着丝粒充当了一条臂进一步运动的屏障。
The formation of the synaptonemal complex (SC) is a crucial early step in the meiotic process, but relatively little is known about the establishment of the human SC. Accordingly, we recently initiated a study of synapsis in the human male, combining immunofluorescence and fluorescence in situ hybridization methodologies to analyze prophase spermatocytes from a series of control individuals. Our results indicate that synapsis is a tightly regulated process, with relatively little variation among individuals. On nonacrocentric chromosomes, there are two synaptic initiation sites, one on the distal short arm and one on the distal long arm, whereas acrocentric chromosomes exhibit a single site on the distal long arm. For both types of chromosomes, synapsis then proceeds toward the centromere, with little evidence that specific p- or q-arm sequences affect the process. However, the centromere appears to have an inhibitory effect on synapsis-that is, when one arm of a nonacrocentric chromosome is "zippered up" before the other, the centromere acts as a barrier to further movement from that arm.