Immobilization of Human Vascular Endothelial Growth Factor (VEGF165) onto Biomaterials: An Evaluation of the Biological Activity of Immobilized VEGF165
Immobilization of Human Vascular Endothelial Growth Factor (VEGF165) onto Biomaterials: An Evaluation of the Biological Activity of Immobilized VEGF165
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DOI：
10.1177/088391150001500403
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发表时间：
2000-07
影响因子：
1.7
通讯作者：
T. Taguchi;A. Kishida;M. Akashi;I. Maruyama
中科院分区：
文献类型：
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作者：
T. Taguchi;A. Kishida;M. Akashi;I. Maruyama
人血管内皮生长因子（VEGF）是诱导特异性内皮细胞增殖的血管生成因子。本研究将含有165个氨基酸的VEGF 165固定在聚丙烯酸接枝聚乙烯膜上。使用水溶性碳二亚胺，通过VEGF 165的氨基与PAAc的羧基之间的反应来固定VEGF 165。以体外培养的人脐静脉内皮细胞（HUVEC）为材料，研究了三种蛋白质在PE膜上的粘附、增殖和迁移情况。使用的蛋白质是胶原蛋白（IV型）、纤连蛋白（FN）和VEGF 165。胶原或FN的固定以及VEGF与FN的共固定均能增强HUVEC的粘附，但单独VEGF不能增强粘附。VEGF FN共固定化表面显示出细胞生长促进活性。仅在胶原固定或VEGF FN共固定的膜表面上观察到内皮化。我们认为，VEGF与FN共固定的生物材料可用于人工血管和其他组织工程支架。
Human vascular endothelial growth factors (VEGF) are angiogenic factors that induce specific endothelial cell proliferation. In this study, VEGF165, which contains 165 amino acids, was immobilized onto poly(acrylic acid) grafted polyethylene) films (PAAc-g-PE). VEGF165 was immobilized via a reaction between the amino group of VEGF165 and the carboxyl group of PAAc using water-soluble carbodiimide. Using cultured human umbilical vein endothelial cells (HUVEC), adhesion, proliferation, and migration of the cells were evaluated with three kinds of protein that were immobilized on the PE films. The proteins used were collagen (type IV), fibronectin (FN), and VEGF165. The adhesion of HUVEC was enhanced by the immobilization of collagen or FN and by the co-immobilization of VEGF with FN, but not VEGF alone. The VEGF FN-co-immobilized surface showed cell growth promotion activity. Endothelialization was observed only on the collagen-immobilized or VEGF FN-co-immobilized film surfaces. We proposed that the VEGF with FN-co-immobilized biomaterials could be used for artificial vessels and for other tissue engineering scaffolds.