The effect of expression levels on codon usage in Plasmodium falciparum
The effect of expression levels on codon usage in Plasmodium falciparum
复制标题
DOI：
10.1017/s0031182003004517
复制
发表时间：
2004-03-01
期刊：
影响因子：
2.4
通讯作者：
Musto, H
中科院分区：
文献类型：
--
作者：
Peixoto, L;Fernández, V;Musto, H
研究了在全测序的、富含cxtrenick-A + T的恶性疟原虫（Plasmodiumfalciparum）中替代同义密码子的使用。重复以前的研究，获得不到3%的总基因最近描述的，我们发现，A-和U-结尾的三联体占主导地位，但翻译选择增加了频率的一个子集的密码子在高表达的基因。然而，一些新的结果来自全序列的分析。首先，GC 3中存在比先前描述的更多的变异;第二，已经用4个不同阶段的真实的表达数据分析了在翻译水平上起作用的自然选择的效果。第三，我们发现高表达的蛋白质增加了能量较低的氨基酸的频率。这些结果的影响进行了讨论。
The usage of alternative synonymous codons in the completely sequenced, cxtrenick- A + T-rich parasite Plasmodium falciparum was Studied. Confirming previous studies obtained with less than 3% of the total genes recently described, we found that A- and U-ending triplets predominate but translational selection increases the frequency of a subset of codons in highly expressed genes. However, some new results come from the analysis of the complete sequence. First, there is more variation in GC3 than previously described; second, the effect of natural selection acting at the level of translation has been analysed with real expression data at 4 different stages mid third, we found that highly expressed proteins increment the frequency of energetically less expensive amino acids. The implications of these results are discussed.