dTRPA1 Modulates Afternoon Peak of Activity of Fruit Flies Drosophila melanogaster.
dTRPA1 Modulates Afternoon Peak of Activity of Fruit Flies Drosophila melanogaster.
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DOI：
10.1371/journal.pone.0134213
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Sheeba V
中科院分区：
文献类型：
--
作者：
Das A;Holmes TC;Sheeba V
半自然条件下果蝇的日常节律受到了广泛的关注。在日照条件下，野生型苍蝇的行为有一个显著的差异，那就是在一天的中午有一个额外的活动高峰。这被称为下午峰（a峰），在使用门控光和温度线索的标准实验室制度下不存在。虽然以前的报告确定了导致a峰的物理因素，但没有证据表明潜在的分子机制或控制a峰的途径。我们报道了a峰是由热敏的dTRPA1（果蝇瞬时受体电位- A1）离子通道介导的，因为在dTRPA1零突变体中没有这个峰。此外，当在实验室中模拟光和温度的自然循环时，我们发现a峰的振幅依赖于dtrpa1。尽管少数昼夜神经元表达dTRPA1，但我们发现a -峰的调节主要受非cry表达dTRPA1的神经元的影响。因此，我们提出在SN下观察到的a -峰活性是果蝇通过dTRPA1受体信号传导引起的温度敏感反应。
Daily rhythms in Drosophila under semi-natural conditions (or SN) have received much recent attention. One of the striking differences in the behaviour of wild type flies under SN is the presence of an additional peak of activity in the middle of the day. This is referred to as the afternoon peak (A-peak) and is absent under standard laboratory regimes using gated light and temperature cues. Although previous reports identified the physical factors that contribute towards the A-peak there is no evidence for underlying molecular mechanisms or pathways that control A-peak. We report that the A-peak is mediated by thermosensitive dTRPA1 (drosophila Transient Receptor Potential- A1) ion channels as this peak is absent in dTRPA1 null mutants. Further, when natural cycles of light and temperature are simulated in the lab, we find that the amplitude of the A-peak is dTRPA1-dependent. Although a few circadian neurons express dTRPA1, we show that modulation of A-peak is primarily influenced by non-CRY dTRPA1 expressing neurons. Hence, we propose that A-peak of activity observed under SN is a temperature sensitive response in flies that is elicited through dTRPA1 receptor signalling.