The protein phosphatase calcineurin is essential for NaCl tolerance of Saccharomyces cerevisiae.
The protein phosphatase calcineurin is essential for NaCl tolerance of Saccharomyces cerevisiae.
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蛋白磷酸酶钙调神经磷酸酶对于酿酒酵母的 NaCl 耐受性至关重要。
DOI：
10.1016/s0021-9258(17)37038-2
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
PardoSn
中科院分区：
文献类型：
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作者：
Imelda MendozaS;Francisco Rubioli;Alonso Rodriguez;José;M.;PardoSn
NaCl敏感的酵母突变体的分离，以确定耐盐性所必需的基因。对Na+和Li+高度敏感的突变体的互补导致CNB 1基因的分离。该基因编码Ca 2 +/钙调蛋白依赖性蛋白磷酸酶钙调磷酸酶的调节亚基（CNB）。CNB缺乏的细胞由于ENA 1的表达减少而积累Li+，ENA 1是一种编码参与Na+和Li+流出的P型ATP酶的基因。此外，cnb 1 δ细胞的K+转运系统没有转化为高亲和力状态，这有助于更好地区分K+和Na+。因此，cnb 1 delta菌株类似于trk 1突变体。这些结果表明，在酿酒酵母适应NaCl胁迫需要一个信号转导途径，涉及Ca 2+和蛋白质磷酸化-去磷酸化。在这一途径中，钙调神经磷酸酶将协调基因表达和离子转运蛋白的活性，以促进离子稳态。
NaCl-sensitive yeast mutants were isolated to identify genes essential for NaCl tolerance. Complementation of a mutant highly sensitive to Na+ and Li+ led to the isolation of the CNB1 gene. This gene encodes the regulatory subunit (CNB) of the Ca2+/calmodulin-dependent protein phosphatase calcineurin. Cells deficient in CNB accumulated Li+ due to reduced expression of ENA1, a gene encoding a P-type ATPase involved in Na+ and Li+ efflux. In addition, the K+ transport system of cnb1 delta cells was not converted to the high affinity state that facilitates better discrimination of K+ over Na+. Thus the cnb1 delta strain resembled a trk1 mutant. These results indicate that adaptation to NaCl stress in Saccharomyces cerevisiae requires a signal transduction pathway involving Ca2+ and protein phosphorylation-dephosphorylation. In this pathway, calcineurin would coordinate gene expression and activity of ion transporters to facilitate ion homeostasis.