Suspended microchannel resonators for biomolecular detection
Suspended microchannel resonators for biomolecular detection
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DOI：
10.1063/1.1611625
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发表时间：
2003-09-29
影响因子：
4
通讯作者：
Manalis, SR
中科院分区：
文献类型：
--
作者：
Burg, TP;Manalis, SR
我们提出了一种共振质量传感器，用于在亚纳升流体体积中检测特定的生物分子。传感原理是基于测量悬浮的微流体通道的共振频率的偏移，分子在装置的内壁上积累。将流体限制在中空悬臂的内部使得能够与传统的微流体系统直接集成，通过消除高阻尼和粘性阻力显著增加灵敏度，并且允许谐振器通过静电力致动。流体密度测量显示在4 mHz-4 Hz带宽内的质量分辨率为10（-17）g/mum（2）。为了证明生物分子检测，我们提出了实时测量亲和素和生物素化牛血清白蛋白之间的特异性结合。基于这些测量，我们预期在优化的系统中可以检测到10（-19）g/mum（2）量级的表面质量负载的变化。(C)2003年，美国物理学会。
We present a resonant mass sensor for specific biomolecular detection in a subnanoliter fluid volume. The sensing principle is based on measuring shifts in resonance frequency of a suspended microfluidic channel upon accumulation of molecules on the inside walls of the device. Confining the fluid to the inside of a hollow cantilever enables direct integration with conventional microfluidic systems, significantly increases sensitivity by eliminating high damping and viscous drag, and allows the resonator to be actuated by electrostatic forces. Fluid density measurements reveal a mass resolution of 10(-17) g/mum(2) in a 4 mHz-4 Hz bandwidth. To demonstrate biomolecular detection, we present real-time measurements of the specific binding between avidin and biotinylated bovine serum albumin. Based on these measurements, we expect that changes in surface mass loading on the order of 10(-19) g/mum(2) can be detected in an optimized system. (C) 2003 American Institute of Physics.