The EcpD Tip Adhesin of the Escherichia coli Common Pilus Mediates Binding of Enteropathogenic E. coli to Extracellular Matrix Proteins.
The EcpD Tip Adhesin of the Escherichia coli Common Pilus Mediates Binding of Enteropathogenic E. coli to Extracellular Matrix Proteins.
复制标题
DOI：
10.3390/ijms231810350
复制
发表时间：
2022-09-08
影响因子：
5.6
通讯作者：
Giron, Jorge A.
中科院分区：
文献类型：
--
作者：
Mondal, Rajesh;Saldana-Ahuactzi, Zeus;Soria-Bustos, Jorge;Schultz, Andrew;Yanez-Santos, Jorge A.;Laguna, Ygnacio Martinez;Cedillo-Ramirez, Maria L.;Giron, Jorge A.
肠致病性大肠杆菌（EPEC）的附着到肠上皮细胞是由几个adhesins促进的，然而，个别宿主细胞的菌毛介导的粘附受体还没有得到充分的表征。在这项研究中，我们评估的假设，E。大肠杆菌普通菌毛（ECP）尖端粘附素蛋白EcpD介导EPEC与几种细胞外基质（ECM）糖蛋白（纤连蛋白、层粘连蛋白、胶原蛋白I和IV以及粘蛋白）的附着。我们发现，ΔecpA突变体，缺乏EcpA丝的生产，但保留在表面上的EcpD，坚持这些糖蛋白低于野生型水平，而ΔecpD突变体，不显示EcpA或EcpD，结合这些宿主糖蛋白显着减少。在协议中，纯化的重组EcpD亚基绑定显着超过EcpA层粘连蛋白，纤连蛋白，胶原蛋白I和IV，粘蛋白的剂量依赖性方式。这些令人信服的数据强烈表明，ECP生产EPEC可能通过尖端粘附素蛋白EcpD结合宿主ECM糖蛋白和粘蛋白。这项研究突出了EPEC的多功能性，以结合不同的主机蛋白质，并表明ECP与主机的ECM糖蛋白的相互作用，可能会促进肠粘膜上皮的定植。
The attachment of enteropathogenic Escherichia coli (EPEC) to intestinal epithelial cells is facilitated by several adhesins; however, the individual host-cell receptors for pili-mediated adherence have not been fully characterized. In this study, we evaluated the hypothesis that the E. coli common pilus (ECP) tip adhesin protein EcpD mediates attachment of EPEC to several extracellular matrix (ECM) glycoproteins (fibronectin, laminin, collagens I and IV, and mucin). We found that the ΔecpA mutant, which lacks production of the EcpA filament but retains EcpD on the surface, adhered to these glycoproteins below the wild-type levels, while the ΔecpD mutant, which does not display EcpA or EcpD, bound significantly less to these host glycoproteins. In agreement, a purified recombinant EcpD subunit bound significantly more than EcpA to laminin, fibronectin, collagens I and IV, and mucin in a dose-dependent manner. These are compelling data that strongly suggest that ECP-producing EPEC may bind to host ECM glycoproteins and mucins through the tip adhesin protein EcpD. This study highlights the versatility of EPEC to bind to different host proteins and suggests that the interaction of ECP with the host’s ECM glycoproteins may facilitate colonization of the intestinal mucosal epithelium.
登录
查看更多内容
影响因子：
9.4
作者：
Blackburn, Dana;Husband, Amanda;Giron, Jorge A.
通讯作者：
Giron, Jorge A.
影响因子：
3.6
作者：
Hartland, EL;Batchelor, M;Frankel, G
通讯作者：
Frankel, G
影响因子：
64.5
作者：
Kenny, B;DeVinney, R;Finlay, BB
通讯作者：
Finlay, BB
影响因子：
4.6
作者：
Geraci J;Neubauer S;Pöllath C;Hansen U;Rizzo F;Krafft C;Westermann M;Hussain M;Peters G;Pletz MW;Löffler B;Makarewicz O;Tuchscherr L
通讯作者：
Tuchscherr L
影响因子：
3.1
作者：
Farfan, Mauricio J.;Cantero, Lidia;Torres, Alfredo G.
通讯作者：
Torres, Alfredo G.