Excretion of West Nile Virus in Urine During Acute Infection
Excretion of West Nile Virus in Urine During Acute Infection
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DOI：
10.1093/infdis/jit290
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发表时间：
2013-10-01
影响因子：
6.4
通讯作者：
Palu, Giorgio
中科院分区：
文献类型：
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作者：
Barzon, Luisa;Pacenti, Monia;Palu, Giorgio
在尿中检测西尼罗河病毒（WNV）RNA已被轶事描述并建议用于诊断WNV感染。本研究报告了常规使用实时逆转录聚合酶链反应检测尿液中的WNV RNA，以支持2012年意大利东北部大爆发期间WNV感染的诊断。32例有症状的WNV感染（定义为神经浸润性疾病和发热）患者中有14例（43.8%）在诊断时尿液中可检测到WNV RNA，其速度和负荷高于血液中WNV RNA的检测，且持续时间更长。在通过WNV核酸扩增检测确定WNV感染的献血者中，尿液中WNV RNA的检测不太常见（14例患者中的2例[14.2%]）。感染性病毒是从一名患有神经侵袭性疾病的患者的尿液中分离出来的，尿液中的西尼罗河病毒RNA含量很高。
Detection of West Nile virus (WNV) RNA in urine has been anecdotally described and proposed for the diagnosis of WNV infection. This study reports the routine use of real-time reverse-transcription polymerase chain reaction for the detection of WNV RNA in urine to support diagnosis of WNV infection during the large outbreak that occurred in northeastern Italy in 2012. Fourteen of 32 patients (43.8%) with symptomatic WNV infection, defined as neuroinvasive disease and fever, had detectable WNV RNA in urine at the time of diagnosis, at a higher rate and load and for a longer time than detection of WNV RNA in blood. Detection of WNV RNA in urine was less frequent (2 of 14 patients [14.2%]) in blood donors in whom WNV infection was identified by WNV nucleic acid amplification testing. Infectious virus was isolated from the urine of a patient with neuroinvasive disease and a high WNV RNA load in urine.