Glucose inhibits development of hamster 8-cell embryos in vitro.
Glucose inhibits development of hamster 8-cell embryos in vitro.
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葡萄糖在体外抑制仓鼠 8 细胞胚胎的发育。
DOI：
10.1095/biolreprod40.3.599
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发表时间：
1989
影响因子：
3.6
通讯作者：
Bavister,BD
中科院分区：
文献类型：
--
作者：
Seshagiri,PB;Bavister,BD
研究了仓鼠8-细胞胚胎体外发育的能量底物（葡萄糖、丙酮酸和乳酸）的相对偏好。使用含有10 mM乳酸盐（L）、0.1 mM丙酮酸盐（P）和氨基酸（Phe、Ile、Met和Gln）的无蛋白质改良台氏培养基（TLP-PVA），我们发现，在不存在葡萄糖的情况下，仓鼠8细胞胚胎发育为囊胚的支持效果优于含有（标准）5 mM葡萄糖的培养基（分别为88.1%和50%）。即使在培养基中添加0.25 mM葡萄糖也能显著抑制囊胚形成（54.1%）。含有5 mM葡萄糖作为唯一能量底物（不含丙酮酸盐、乳酸盐和氨基酸）的培养基T-PVA对胚胎发育的支持非常差（< 7.9%囊胚），但添加0.1 mM丙酮酸盐可增强囊胚形成（52%）。消除丙酮酸在TL-PVA培养基中含有5 mM葡萄糖和氨基酸显着减少囊胚形成4倍（13.5%），最佳的丙酮酸浓度为0.2 mM。然而，如果相同的介质是缺乏葡萄糖，囊胚形成高的情况下（71.1%）和存在（83.3%）的0.1 mM丙酮酸。同样，在无葡萄糖的T-PVA培养基中，添加10 mM乳酸盐或氨基酸支持8细胞胚胎发育为囊胚（分别为61.7%和60.5%），而在5 mM葡萄糖的存在下，分别为18.8%和30.6%。这种增强的发展，在没有葡萄糖的情况下，建议由于乳酸的有效转化为丙酮酸和氨基酸的两用中间体，因此它们的利用通过克雷布斯循环。葡萄糖本身不仅抑制发育，而且抑制乳酸/氨基酸介导的发育。这些结果表明，仓鼠8细胞胚胎采用克雷布斯循环，而不是糖酵解途径，为他们的主要能源生产过程中的发展。这些数据揭示了与小鼠胚胎相比，仓鼠8细胞胚胎的能量底物需求存在显着差异。
Relative preferences of energy substrates (glucose, pyruvate, and lactate) for in vitro development of hamster 8-cell embryos were investigated. Using protein-free modified Tyrode’s medium (TLP-PVA) containing 10 mM lactate (L), 0.1 mM pyruvate (P), and amino acids (Phe, Ile, Met and Gln), we found that development of hamster 8-cell embryos to blastocysts was supported better in the absence of glucose than in medium containing (standard) 5 mM glucose (88.1% and 50%, respectively). Addition of even 0.25 mM glucose to the medium significantly inhibited blastocyst formation (54.1%). Medium T-PVA, containing 5 mM glucose as sole energy substrate (without pyruvate, lactate, and amino acids), very poorly supported embryo development (< 7.9% blastocysts), but addition of 0.1 mM pyruvate enhanced blastocyst formation (52%). Elimination of pyruvate in TL-PVA medium containing 5 mM glucose and amino acids markedly reduced blastocyst formation by 4-fold (13.5%); the optimal pyruvate concentration was 0.2 mM. However, if the same medium was devoid of glucose, blastocyst formation was high both in the absence (71.1%) and presence (83.3%) of 0.1 mM pyruvate. Similarly, in glucose-free T-PVA medium, addition of either 10 mM lactate or amino acids supported 8-cell embryo development to blastocysts (61.7% and 60.5%, respectively) as opposed to 18.8% and 30.6%, respectively, in the presence of 5 mM glucose. This augmented development in the absence of glucose is suggested to the due to the efficient conversion of lactate to pyruvate and of amino acids to amphibolic intermediates and hence their utilization via the Krebs cycle. Glucose by itself not only inhibited development but also inhibited lactate/amino acidsmediated development. These results imply that hamster 8-cell embryos employ the Krebs cycle, not the glycolytic pathway, for their major energy production during development. The data reveal striking differences in the energy substrate requirements of hamster 8-cell embryos compared with mouse embryos.