HIV-1-infected CD8+CD4+ T cells decay in vivo at a similar rate to infected CD4 T cells during HAART
HIV-1-infected CD8+CD4+ T cells decay in vivo at a similar rate to infected CD4 T cells during HAART
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DOI：
10.1097/qad.0b013e3282f151b9
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发表时间：
2008-01-02
期刊：
影响因子：
3.8
通讯作者：
Simmonds, Peter
中科院分区：
文献类型：
--
作者：
Hughes, Gareth J.;Cochrane, Alexandra;Simmonds, Peter
目的：通过测量感染的CD 4/CD 8 DP T细胞的衰变率，研究HAART后CD 4 + CD 8 + T细胞[CD 8双阳性（CD 8 DP）] T细胞形成HIV-1储库的潜力。在治疗200-400天之前和之后，测定研究受试者的高纯度CD 4和CD 8 DP T细胞中的HIV-1前病毒载量，结果：治疗前CD 8 DP中HIV-1前病毒载量与CD 4细胞计数呈负相关。HIV-1感染的CD 4和CD 8 DP T细胞的衰变率相似。CD 8 DP T细胞的比率与抑制病毒复制的时间相关，而CD 4细胞衰减率则没有这种关系。观察到两种细胞类型的活化细胞显著减少。HAART对HIV-1复制的作用对于CD 4细胞和CD 8 DP T细胞是相似的，尽管感染的CD 8 DP T细胞的清除率对于血浆病毒载量的快速降低似乎更关键。虽然在外周血中的CD 8 DP T细胞水库的大小是较小的相对于CD 4细胞，HAART没有完全清除HIV-1感染从这个细胞subset.Conclusion：这项研究证实，CD 8 DP T细胞面积主要水库HIV-1在体内，因此，代表一个潜在的水库HIV-1在HAART期间，以类似的方式的CD 4 T细胞。(c)2008年威科健康。
Objectives: To investigate the potential for CD4+CD8+ T cells [CD8 double positive (CD8 DP)] T cells to form a reservoir of HIV-1 following HAART through measurement of the rate of decay of infected CD4/CD8 DP T cells.Methods: HIV-1 proviral loads in highly pure CD4 and CD8 DP T cells were determined for study subjects before and after 200-400 days of therapy and HIV-1 DNA decay rates were calculated.Results: Before therapy, HIV-1 proviral load in CD8 DP correlated negatively with CD4 cell count. Decay rates of HIV-1-infected CD4 and CD8 DP T cells were similar. Rates for CD8 DP T cells correlated with the time to suppression of viral replication, whereas no such relationship was true for CD4 cell decay rates. A significant reduction in activated cells was observed for both cell types. The action of HAART on HIV-1 replication was similar for both CD4 cells and CD8 DP T cells, although the rate of clearance of infected CD8 DP T cells appeared more critical for a rapid reduction in plasma viral load. Although the size of the CD8 DP T cell reservoir in peripheral blood was smaller relative to that of CD4 cells, HAART did not completely clear HIV-1 infection from this cell subset.Conclusion: This study confirmed that CD8 DP T cells area major reservoir for HIV-1 in vivo and, therefore, represent a potential reservoir for HIV-1 during HAART, in a manner analogous to that of CD4 T cells. (c) 2008 Wolters Kluwer Health.