Patterns of hippocampal cell loss based on subregional lesions of the hippocampus.
Patterns of hippocampal cell loss based on subregional lesions of the hippocampus.
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基于海马次区域病变的海马细胞丢失模式。
DOI：
10.1016/j.brainres.2005.09.062
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Berman,RobertF
中科院分区：
文献类型：
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作者：
Jerman,TaylorS;Kesner,RaymondP;Lee,Inah;Berman,RobertF
人们普遍认为海马体在记忆中起着至关重要的作用。此外，研究表明，海马内的子区域对记忆的特定行为成分有不同的贡献。这些研究通常依赖于局部注射神经毒素（例如，鹅膏蕈氨酸或秋水仙碱）进入海马的靶向亚区。在本研究中，鹅膏蕈氨酸病变到CA 1和CA 3区和秋水仙素病变到背侧齿状回（DG）的特异性进行了测试。具体而言，背侧海马、腹侧海马和海马外区域（例如，外侧隔和内嗅皮质）使用Fluoro-Jade进行评价，Fluoro-Jade是一种用于变性神经元的组织荧光染色。结果表明，鹅膏蕈氨酸注射到CA 1和CA 3区后细胞损失相对均匀，而秋水仙素注射到DG区后细胞损失变化不定。CA 1和CA 3病变主要局限于这些相关亚区，DG病变高度局限于DG。使用这些损伤程序，在腹侧海马中几乎没有明显的细胞损失，在内嗅皮层中没有明显的细胞损失。有人建议，在这项研究中所描述的病变程序产生相对选择性病变的神经元内的特定子区域的海马，应该是有用的研究检查可能的差异贡献海马子区域的记忆过程。
It is widely accepted that the hippocampus plays an essential role in memory. Furthermore, studies have suggested that subregions within the hippocampus contribute differentially to specific behavioral components of memory. These studies typically rely on lesions produced by localized injections of neurotoxins (e.g., ibotenic acid or colchicine) into targeted subregions of the hippocampus. In the present study, the specificity of ibotenic acid lesions into areas CA1 and CA3 and colchicine lesions into the dorsal dentate gyrus (DG) was tested. Specifically, the effects of lesions within the dorsal hippocampus, the ventral hippocampus, and areas outside the hippocampus (e.g., lateral septum and entorhinal cortex) were evaluated using Fluoro-Jade, a histofluorescent stain for degenerating neurons. The results show that cell loss is relatively uniform after ibotenic acid injections into areas CA1 and CA3 and variable after colchicine injections into DG. CA1 and CA3 lesions appeared mostly localized to those relative subregions, and DG lesions appeared highly localized to the DG. Using these lesion procedures, little cell loss was apparent in the ventral hippocampus, and no cell loss was apparent in the entorhinal cortex. It is suggested that the lesion procedures described in this study produce relatively selective lesions of neurons within specific subregions of the hippocampus and should be useful for studies examining possible differential contributions of hippocampal subregions to memory processes.