EXPERIMENTAL PHYLOGENETICS - GENERATION OF A KNOWN PHYLOGENY
EXPERIMENTAL PHYLOGENETICS - GENERATION OF A KNOWN PHYLOGENY
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DOI：
10.1126/science.1736360
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发表时间：
1992-01-31
期刊：
影响因子：
56.9
通讯作者：
MOLINEUX, IJ
中科院分区：
文献类型：
--
作者：
HILLIS, DM;BULL, JJ;MOLINEUX, IJ
虽然系统发育估计方法在比较生物学中被常规使用，但由于缺乏已知的系统发育，这些方法的直接测试受到阻碍。在这里，基于在诱变剂存在下噬菌体T7的连续繁殖的系统被用于创建第一个完全已知的突变体。终端谱系的限制位点图被用来推断实验品系的进化历史，以与已知的历史和实际的祖先进行比较。用于重建分支模式的五种方法都预测了正确的拓扑结构，但在预测分支长度方面有所不同;一种方法还预测了祖先的限制性内切酶图谱，发现其准确率超过98%。
Although methods of phylogenetic estimation are used routinely in comparative biology, direct tests of these methods are hampered by the lack of known phylogenies. Here a system based on serial propagation of bacteriophage T7 in the presence of a mutagen was used to create the first completely known phylogeny. Restriction-site maps of the terminal lineages were used to infer the evolutionary history of the experimental lines for comparison to the known history and actual ancestors. The five methods used to reconstruct branching pattern all predicted the correct topology but varied in their predictions of branch lengths; one method also predicts ancestral restriction maps and was found to bc greater than 98 percent accurate.