A Eukaryotic-like Serine/Threonine Kinase Protects Staphylococci against Phages
A Eukaryotic-like Serine/Threonine Kinase Protects Staphylococci against Phages
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DOI：
10.1016/j.chom.2016.08.010
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发表时间：
2016-10-12
影响因子：
30.3
通讯作者：
Bikard, David
中科院分区：
文献类型：
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作者：
Depardieu, Florence;Didier, Jean-Philippe;Bikard, David
来自生命各个领域的生物体都受到病毒的感染。在真核生物中，丝氨酸/苏氨酸激酶在抗病毒反应中起核心作用。然而，细菌通常不知道使用蛋白质磷酸化作为其防御细菌的一部分。在这里，我们确定Stk 2，葡萄球菌丝氨酸/苏氨酸激酶，提供有效的免疫噬菌体诱导流产感染。一种功能未知的噬菌体蛋白激活Stk 2激酶。这导致Stk 2依赖性磷酸化的几种蛋白质参与翻译，全球转录控制，细胞周期控制，应激反应，DNA拓扑结构，DNA修复和中央代谢。细菌宿主细胞由于Stk 2活化而死亡，从而阻止噬菌体繁殖到细菌群体的其余部分。我们的工作表明，依赖于蛋白磷酸化的病毒防御机制构成了一种跨越生命多个领域的保守的抗病毒策略。
Organisms from all domains of life are infected by viruses. In eukaryotes, serine/threonine kinases play a central role in antiviral response. Bacteria, however, are not commonly known to use protein phosphorylation as part of their defense against phages. Here we identify Stk2, a staphylococcal serine/threonine kinase that provides efficient immunity against bacteriophages by inducing abortive infection. A phage protein of unknown function activates the Stk2 kinase. This leads to the Stk2-dependent phosphorylation of several proteins involved in translation, global transcription control, cell-cycle control, stress response, DNA topology, DNA repair, and central metabolism. Bacterial host cells die as a consequence of Stk2 activation, thereby preventing propagation of the phage to the rest of the bacterial population. Our work shows thatmechanisms of viral defense that rely on protein phosphorylation constitute a conserved antiviral strategy across multiple domains of life.