Salicylic acid-based poly(anhydride esters) for control of biofilm formation in Salmonella enterica serovar Typhimurium
Salicylic acid-based poly(anhydride esters) for control of biofilm formation in Salmonella enterica serovar Typhimurium
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DOI：
10.1111/j.1472-765x.2008.02356.x
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发表时间：
2008-05-01
影响因子：
2.4
通讯作者：
Chikindas, M. L.
中科院分区：
文献类型：
--
作者：
Rosenberg, L. E.;Carbone, A. L.;Chikindas, M. L.
目的：与游离浮游细胞相比，细菌生物膜通常具有更强的抗压力能力。因此，发现对细菌生物膜形成具有强烈抑制作用的抗菌应激因子，将对食品、个人护理和医疗行业产生重大影响。方法和结果：水杨酸基聚酸酐酯（PAE）先前已被证明可能通过影响表面附着来抑制生物膜的形成。我们的研究评估了水杨酸基PAE对形成生物膜的肠沙门氏菌血清型鼠伤寒杆菌的影响。为了去除与表面物理和化学参数相关的因素，我们使用了一种在气液界面形成生物膜的菌株。表面特性会影响生物膜的特性，因此不依附于固体表面消除了这些限制。结果表明，水杨酸基聚合物确实会干扰生物膜的形成，因为在空气-液体界面（顶部形成）和在表面-液体界面（底部形成）形成生物膜的细菌菌株之间存在明显的差异。结论：这些结果表明聚合物可能不会干扰附着；相反，聚合物可能会影响沙门氏菌生物膜形成的另一种重要机制。研究的意义和影响：生物膜的形成可以通过控制释放天然来源的抗菌剂配制到聚合物系统中来防止。
Aims: Bacterial biofilms generally are more resistant to stresses as compared with free planktonic cells. Therefore, the discovery of antimicrobial stress factors that have strong inhibitory effects on bacterial biofilm formation would have great impact on the food, personal care, and medical industries.Methods and Results: Salicylate-based poly(anhydride esters) (PAE) have previously been shown to inhibit biofilm formation, possibly by affecting surface attachment. Our research evaluated the effect of salicylate-based PAE on biofilm-forming Salmonella enterica serovar Typhimurium. To remove factors associated with surface physical and chemical parameters, we utilized a strain that forms biofilms at the air-liquid interface. Surface properties can influence biofilm characteristics, so the lack of attachment to a solid surface eliminates those constraints. The results indicate that the salicylic acid-based polymers do interfere with biofilm formation, as a clear difference was seen between bacterial strains that form biofilms at the air-liquid interface (top-forming) and those that form at the surface-liquid interface (bottom-forming).Conclusion: These results lead to the conclusion that the polymers may not interfere with attachment; rather, the polymers likely affect another mechanism essential for biofilm formation in Salmonella.Significance and Impact of the study: Biofilm formation can be prevented through controlled release of nature-derived antimicrobials formulated into polymer systems.