Circulating Progenitor Cell Response to Exercise in Wheelchair Racing Athletes
Circulating Progenitor Cell Response to Exercise in Wheelchair Racing Athletes
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DOI：
10.1249/mss.0000000000001402
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发表时间：
2018-01-01
期刊：
影响因子：
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通讯作者：
De Lisio, Michael
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文献类型：
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作者：
Niemiro, Grace M.;Edwards, Thomas;De Lisio, Michael
循环祖细胞（CPC）是外周血中参与组织修复的干/祖细胞的异质群体。CPC动员已在健全人中得到很好的表征，但以前没有在轮椅赛车运动员中进行过研究。本研究的目的是描述精英轮椅赛车运动员在急性上肢有氧运动中CPC和CPC亚群动员的特征，以确定CPC反应是否与非卧床人群相似。（3名女性;年龄= 27.5 ± 4.0岁，仰卧位身高= 162.5 ± 18.6 cm，体重= 53.5 +/- 10.9 kg，VO 2峰值= 2.4 +/- 0.62 Lmin（-1），年= 21.5 +/- 6.2年）完成了25公里的公路计时赛。在运动前和运动后立即进行血液采样，用于定量CPC（CD 34（+））、造血干细胞和祖细胞（HSPC）（CD 34（+）/CD 45（dim））、造血干细胞（HSC）（CD 34（+）/CD 45（dim）/CD 38（-）），CD 34（+）脂肪组织（AT）来源的间充质基质细胞（MSC）（CD 45（-）/CD 34（+）/CD 105（+）/CD 31（-）），CD 34（-）骨髓（BM）来源的MSC（CD 45（-）/CD 34（-）/CD 105（+）/CD 31（-））和内皮祖细胞（EPC）（CD 45（dim）/CD 34（+）/VEGFR 2（+））。结果运动后CPC浓度增加了5.7倍（P = 0.10）。HSPC、HSC、EPC和两种MSC群体在运动后均未增加。基线HSPC人群与绝对VO 2峰值显著正相关（rho = 0.71，P < 0.05），HSC倾向于与VO 2峰值正相关（rho = 0.62，P = 0.10）。AT-MSC群体倾向于与基线VO 2峰值呈负相关（rho =-0.62，P = 0.058）。运动前后CPC、EPC和AT-MSC的变化与乳酸浓度的变化呈显著正相关（rho = 0.91 P = 0.002，0.71 P = 0.047，0.81 P = 0.02，均P < 0.05）。结论轮椅竞速运动员CPC含量与心肺功能有关，对运动的反应与运动强度呈正相关。
Introduction Circulating progenitor cells (CPC) are a heterogeneous population of stem/progenitor cells in peripheral blood that participate in tissue repair. CPC mobilization has been well characterized in able-bodied persons but has not been previously investigated in wheelchair racing athletes. The purpose of this study was to characterize CPC and CPC subpopulation mobilization in elite wheelchair racing athletes in response to acute, upper-extremity aerobic exercise to determine whether CPC responses are similar to ambulatory populations.Methods Eight participants (three females; age = 27.5 4.0 yr, supine height = 162.5 18.6 cm, weight = 53.5 +/- 10.9 kg, VO2peak = 2.4 +/- 0.62 Lmin(-1), years postinjury = 21.5 +/- 6.2 yr) completed a 25-km time trial on a road course. Blood sampling occurred before and immediately after exercise for quantification of CPC (CD34(+)), hematopoietic stem and progenitor cells (HSPC) (CD34(+)/CD45(dim)), hematopoietic stem cells (HSC) (CD34(+)/CD45(dim)/CD38(-)), CD34(+) adipose tissue (AT)-derived mesenchymal stromal cells (MSC) (CD45(-)/CD34(+)/CD105(+)/CD31(-)), CD34(-) bone marrow (BM)-derived MSC (CD45(-)/CD34(-)/CD105(+)/CD31(-)), and endothelial progenitor cells (EPC) (CD45(dim)/CD34(+)/VEGFR2(+)) via flow cytometry. Blood lactate was measured before and after trial as an indicator of exercise intensity.Results CPC concentration increased 5.7-fold postexercise (P = 0.10). HSPC, HSC, EPC, and both MSC populations were not increased postexercise. Baseline HSPC populations were significantly positively correlated to absolute VO2peak (rho = 0.71, P < 0.05) with HSC trending to positively correlate to VO2peak (rho = 0.62, P = 0.10). AT-MSC populations were trending to be negatively correlated to baseline VO2peak (rho = -0.62, P = 0.058). The change in CPC, EPC, and AT-MSC pre- and postexercise significantly positively correlated to the change in lactate concentrations (rho = 0.91 P = 0.002, 0.71 P = 0.047, 0.81 P = 0.02, respectively, all P < 0.05).Conclusion These data suggest that CPC content in wheelchair racing athletes is related to cardiorespiratory fitness, and responses to exercise are positively related to exercise intensity.