LIPOFUSCIN - RESOLUTION OF DISCREPANT FLUORESCENCE DATA
LIPOFUSCIN - RESOLUTION OF DISCREPANT FLUORESCENCE DATA
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DOI：
10.1126/science.7079738
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发表时间：
1982-01-01
期刊：
影响因子：
56.9
通讯作者：
FEENEYBURNS, L
中科院分区：
文献类型：
--
作者：
ELDRED, GE;MILLER, GV;FEENEYBURNS, L
在荧光显微镜下，脂褐素颗粒（年龄色素）在紫外线激发下发出黄色光。所报道的载脂褐素细胞提取物的蓝色发射最大值可能是仪器偏倚所致。随着年龄的增长，在这些人视网膜色素上皮的溶酶体残留体中积累的主要荧光成分是发黄色荧光团。不同的与年龄相关的荧光团，发出蓝光，来自其他细胞内来源。有必要重新评估蓝色脂质过氧化产物和经典病理学中与年龄相关的脂褐素颗粒之间的联系。
Lipofuscin granules (age pigments) emit yellow light under UV excitation in the fluorescence microscope. The reported blue emission maximum of extracts of lipofuscin-laden cells may result from instrumental bias. The major fluorescent components that accumulate with age in these lysosomal residual bodies of human retinal pigment epithelium are yellow-emitting fluorophores. Different age-related fluorophores, which do emit blue light, are derived from other intracellular sources. A reevaluation of the connection between blue-emitting lipid peroxidation products and the age-related lipofuscin granules of classical pathology is necessary.