MOLECULAR WEIGHTS OF COLI PHAGES AND COLI PHAGE DNA PART 1 MEASUREMENT OF THE MOLECULAR WEIGHT OF BACTERIO PHAGE T-7 BY HIGH SPEED EQUILIBRIUM CENTRIFUGATION
MOLECULAR WEIGHTS OF COLI PHAGES AND COLI PHAGE DNA PART 1 MEASUREMENT OF THE MOLECULAR WEIGHT OF BACTERIO PHAGE T-7 BY HIGH SPEED EQUILIBRIUM CENTRIFUGATION
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DOI：
10.1016/0022-2836(70)90124-5
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发表时间：
1970-01-01
影响因子：
5.6
通讯作者：
FREIFELDER D
中科院分区：
文献类型：
--
作者：
BANCROFT F C;FREIFELDER D
Yphantis（1964）的高速平衡离心法的适用范围已扩大到测定大肠杆菌噬菌体T7的分子量。通过测比重法获得噬菌体T7、T5和T4的浓度值;通过测量氮和磷含量以及氨基酸和核苷酸组成来确定噬菌体浓度。\ldgn值为：T7，0.639; T5，0.658和T4，0.618。根据氮和磷的测量，计算了每种噬菌体类型中的DNA百分比。这些值为：T7，51.2; T5，61.7和T4，54.9。T7噬菌体和T7 DNA的分子量分别为49.4和25.3百万道尔顿。在下面的论文（Dubin，Benedek，班克罗夫特和Freifelder，1970）中，报道了通过另一种技术测定的T7、T5和T4的分子量的值。由这两种技术得到的T7分子量的值非常一致。Freifelder（1970）讨论了分子量测量的一般问题。
The range of usefulness of the high-speed equilibrium centrifugation method of Yphantis (1964) has been extended to measure the molecular weight of Escherichia coli phage T7. Values for\̄ gn of phages T7, T5 and T4 were obtained by pycnometry; the phage concentrations were determined by measuring nitrogen and phosphorus contents, and the amino-acid and nucleotide composition. The\̄ gn values are: T7, 0.639; T5, 0.658 and T4, 0.618. From the nitrogen and phosphorus measurements, the percentage DNA in each phage type has been calculated. These values are: T7, 51.2; T5, 61.7 and T4, 54.9. The molecular weights of T7 phage and T7 DNA are 49.4 and 25.3 million daltons, respectively. In the following paper (Dubin, Benedek, Bancroft & Freifelder, 1970), values are reported of the molecular weights of T7, T5 and T4, determined by another technique. The values yielded by the two techniques for the molecular weight of T7 are in excellent agreement. Discussion of the general problem of molecular weight measurement is given in Freifelder (1970).‡