In Situ, Noninvasive, T2*-Weighted MRI-Derived Parameters Predict Ex Vivo Structural Properties of an Anterior Cruciate Ligament Reconstruction or Bioenhanced Primary Repair in a Porcine Model
In Situ, Noninvasive, T2*-Weighted MRI-Derived Parameters Predict Ex Vivo Structural Properties of an Anterior Cruciate Ligament Reconstruction or Bioenhanced Primary Repair in a Porcine Model
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DOI：
10.1177/0363546512472978
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发表时间：
2013-03-01
影响因子：
4.8
通讯作者：
Fleming, Braden C.
中科院分区：
文献类型：
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作者：
Biercevicz, Alison M.;Miranda, Daniel L.;Fleming, Braden C.
背景资料：磁共振成像（MRI）是一种非侵入性技术，可以定量评估前交叉韧带（ACL）移植物的大小和信号强度。然而，在愈合过程中这些特性与重建或修复韧带强度的关系尚不清楚。假设：磁共振成像衍生的移植物体积和信号强度的测量是ACL或ACL移植物在愈合15周和52周后结构特性的重要预测因素。研究设计：对照实验室研究。方法：目前的数据收集自2项评价ACL重建和修复技术的实验。在第一个实验中，猪进行了单侧ACL横断，并接受（1）ACL重建，（2）ACL重建与胶原-血小板复合物（CPC），或（3）没有治疗。愈合15周后取出手术腿。在第二个实验中，猪进行ACL横断并接受（1）ACL重建，（2）使用CPC的ACL重建，（3）使用CPC的生物增强ACL初次修复，或（4）不治疗。52周后取出手术腿。使用T2* 加权三维稳态相长干涉（CISS）序列对采集的膝关节进行成像。从扫描中分割出每条韧带，并确定关节内体积和中值灰度值。结果：体积显著预测韧带和移植物的结构特性（最大载荷、屈服载荷和线性刚度）（R-2分别为0.56、0.56和0.49; P
Background: Magnetic resonance imaging (MRI) is a noninvasive technology that can quantitatively assess anterior cruciate ligament (ACL) graft size and signal intensity. However, how those properties relate to reconstructed or repaired ligament strength during the healing process is yet unknown.Hypothesis: Magnetic resonance imaging-derived measures of graft volume and signal intensity are significant predictors of the structural properties of an ACL or ACL graft after 15 weeks and 52 weeks of healing.Study Design: Controlled laboratory study.Methods: The current data were gathered from 2 experiments evaluating ACL reconstruction and repair techniques. In the first experiment, pigs underwent unilateral ACL transection and received (1) ACL reconstruction, (2) ACL reconstruction with collagen-platelet composite (CPC), or (3) no treatment. The surgical legs were harvested after 15 weeks of healing. In the second experiment, pigs underwent ACL transection and received (1) ACL reconstruction, (2) ACL reconstruction with CPC, (3) bioenhanced ACL primary repair with CPC, or (4) no treatment. The surgical legs were harvested after 52 weeks. The harvested knees were imaged using a T2*-weighted 3-dimensional constructive interference in steady state (CISS) sequence. Each ligament was segmented from the scans, and the intra-articular volume and the median grayscale values were determined. Mechanical testing was performed to establish the ligament structural properties.Results: Volume significantly predicted the structural properties (maximum load, yield load, and linear stiffness) of the ligaments and grafts (R-2 = 0.56, 0.56, and 0.49, respectively; P