Ligation-mediated PCR for chromatin-structure analysis of interphase and metaphase chromatin
Ligation-mediated PCR for chromatin-structure analysis of interphase and metaphase chromatin
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DOI：
10.1006/meth.1996.0412
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发表时间：
1997-02-01
期刊：
影响因子：
4.8
通讯作者：
Riggs, AD
中科院分区：
文献类型：
--
作者：
Hershkovitz, M;Riggs, AD
染色质结构对于充分理解基因功能和细胞记忆的重要性日益被认识。在细胞记忆中，染色质编码的表观遗传信息从一代传递到另一代，中期染色质的详细结构至关重要。在本文中，我们描述了使用硫酸二甲酯、dna酶I和高锰酸钾进行体内足迹和染色质分析的方法，特别强调了中期细胞的研究。我们回顾了使用连接介导的PCR分析染色质，包括人磷酸甘油酸激酶启动子，并报告了初步研究的基质附着区域附近的人β -干扰素基因。(C) 1997学术出版社。
Chromatin structure is becoming increasingly recognized as important for a full understanding of gene function and cell memory. With regard to cell memory, which involves the transfer of chromatin-encoded epigenetic information from one cell generation to another, the detailed structure of metaphase chromatin is of crucial importance. In this paper we describe methods for the use of dimethyl sulfate, DNase I, and potassium permanganate for in vivo footprinting and chromatin analysis, with special emphasis on studies of metaphase cells. We review the use of ligation-mediated PCR for the analysis of chromatin, including the human phosphoglycerate kinase promoter, and also report initial studies of a matrix attachment region near the human beta-interferon gene. (C) 1997 Academic Press.