Insights into hypoxic systemic responses based on analyses of transcriptional regulation in Arabidopsis.
Insights into hypoxic systemic responses based on analyses of transcriptional regulation in Arabidopsis.
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DOI：
10.1371/journal.pone.0028888
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Shih MC
中科院分区：
文献类型：
--
作者：
Hsu FC;Chou MY;Peng HP;Chou SJ;Shih MC
我们采用了缺氧处理系统，其中只有根处于缺氧条件下。通过分析芽和根的全局转录变化，我们发现系统信号可能从根转导触发非直接缺氧组织的反应。洪水下这种系统反应的分子机制目前在很大程度上是未知的。通过对受调控基因的本体论分类，观察到碳水化合物代谢的系统性管理程序，为系统反应如何促进植物在洪水下的生存提供了一个例子。此外，一个乙烯信号突变ein2-5也影响了在茎部受水淹调控的基因表达比例。许多参与系统性碳水化合物管理程序、激素反应和代谢、泛素依赖蛋白降解的系统反应基因也在ein2-5中受到影响。这些结果表明乙烯在缺氧系统反应的介导中起重要作用。与脱落酸（ABA）生物合成相关的基因在茎中表达上调，在根中表达下调。ABA信号突变，abi1 -1，影响几个系统反应基因的表达。这些结果表明，ABA的生物合成可能需要调节系统反应。讨论了这些结果对根淹拟南芥系统反应的影响。
We have adopted a hypoxic treatment system in which only roots were under hypoxic conditions. Through analyzing global transcriptional changes in both shoots and roots, we found that systemic signals may be transduced from roots to trigger responses in tissues not directly subjected to hypoxia. The molecular mechanisms of such systemic responses under flooding are currently largely unknown. Using ontological categorization for regulated genes, a systemic managing program of carbohydrate metabolism was observed, providing an example of how systemic responses might facilitate the survival of plants under flooding. Moreover, a proportion of gene expressions that regulated in shoots by flooding was affected in an ethylene signaling mutation, ein2-5. Many systemic-responsive genes involved in the systemic carbohydrate managing program, hormone responses and metabolism, ubiquitin-dependent protein degradation were also affected in ein2-5. These results suggested an important role of ethylene in mediation of hypoxic systemic responses. Genes associated with abscisic acid (ABA) biosynthesis are upregulated in shoots and down regulated in roots. An ABA signaling mutation, abi4-1, affects expression of several systemic responsive genes. These results suggested that regulation of ABA biosynthesis could be required for systemic responses. The implications of these results for the systemic responses of root-flooded Arabidopsis are discussed.
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