Flower Forms an Extracellular Code that Reveals the Fitness of a Cell to its Neighbors in Drosophila
Flower Forms an Extracellular Code that Reveals the Fitness of a Cell to its Neighbors in Drosophila
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DOI：
10.1016/j.devcel.2010.05.010
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发表时间：
2010-06-15
影响因子：
11.8
通讯作者：
Moreno, Eduardo
中科院分区：
文献类型：
--
作者：
Rhiner, Christa;Lopez-Gay, Jesus M.;Moreno, Eduardo
细胞竞争促进了从不断增长的群体中淘汰较弱的细胞。在这里，我们调查如何果蝇翅成虫盘细胞区分“赢家”从“输家”在细胞竞争。使用基因组和功能分析，我们已经确定了几个因素参与的过程中，包括花（Fwe），细胞膜蛋白保守的多细胞动物。我们的研究结果表明，Fwe是细胞竞争反应的一个组成部分，它是必要的，足以将细胞标记为“赢家”或“输家”。在果蝇中，fwe基因座产生三种亚型，fwe（ubi），fwe（Lose-A）和fwe（Lose-B）。基础水平的fwe（ubi）不断产生。在竞争期间，fwe（Lose）亚型在潜在的失败者细胞中上调。相对fwe（Lose）和fwe（ubi）水平的细胞-细胞比较最终确定哪个细胞经历凋亡。这种“细胞外密码”可能构成了终止细胞间竞争冲突的古老机制。
Cell competition promotes the elimination of weaker cells from a growing population. Here we investigate how cells of Drosophila wing imaginal discs distinguish "winners" from "losers" during cell competition. Using genomic and functional assays, we have identified several factors implicated in the process, including Flower (Fwe), a cell membrane protein conserved in multicellular animals. Our results suggest that Fwe is a component of the cell competition response that is required and sufficient to label cells as "winners" or "losers." In Drosophila, the fwe locus produces three isoforms, fwe(ubi), fwe(Lose-A), and fwe(Lose-B). Basal levels of fwe(ubi) are constantly produced. During competition, the fwe(Lose) isoforms are upregulated in prospective loser cells. Cell-cell comparison of relative fwe(Lose) and fwe(ubi) levels ultimately determines which cell undergoes apoptosis. This "extracellular code" may constitute an ancient mechanism to terminate competitive conflicts among cells.