Comparison of possible arrangements of five identical columns in preparative chromatography.

Comparison of possible arrangements of five identical columns in preparative chromatography.
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制备色谱中五个相同柱的可能排列比较

DOI:
10.1016/j.chroma.2006.03.065
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发表时间:
2006
期刊:
Journal of chromatography. A
影响因子:
--
通讯作者:
A. Seidel-Morgenstern
A. Seidel-Morgenstern
中科院分区:
--
文献类型:
--
作者:
G. Ziomek;D. Antos;L. Tobiska;A. Seidel-Morgenstern

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本文的目的是证明一定数量的相同 HPLC 色谱柱的配置如何影响目标成分分离的有效性。有两种极端配置:所有可用的色谱柱并联运行(假设有足够的可用泵)或所有色谱柱串联。并联和串联连接的柱的各种组合是可能的。除了批量操作之外,还考虑了允许连续分离的更复杂的多柱布置。特别是,在这项工作中,检查了五根柱的不同配置。为了说明,考虑了二元混合物的分离。作为目标函数,已使用保留较少的组分的生产率。能够描述色谱带传播的标准模型已用于优化各种可能的多柱排列的操作条件。
Goal of the paper is to demonstrate how configuration of a certain number of identical HPLC columns influences on the effectiveness of the isolation of a target component. There are two extreme configurations: all the available columns are operated in parallel (provided there are enough pumps available) or all the columns are connected in series. Various combinations of columns connected parallel and in series are possible. Besides batch operation more sophisticated multicolumn arrangements allowing continuous separation are considered. In particular, in this work different configurations of five columns were examined. For illustration the separation of a binary mixture was considered. As the objective function the productivity of the less retained component has been used. A standard model capable to describe the propagation of chromatographic bands has been used to optimize the operating conditions for various possible multicolumn arrangements.
DOI: 10.1016/0009-2509(85)85001-6
发表时间: 1985
影响因子: 4.7
作者:
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通讯作者: D. Ruthven
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