Comparative analysis of mouse DSP and DPP expression in odontoblasts, preameloblasts, and experimentally induced odontoblast-like cells
Comparative analysis of mouse DSP and DPP expression in odontoblasts, preameloblasts, and experimentally induced odontoblast-like cells
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DOI：
10.1111/j.1600-0722.1998.tb02184.x
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发表时间：
1998-01-01
影响因子：
1.9
通讯作者：
Butler, WT
中科院分区：
文献类型：
--
作者：
Bègue-Kirn, C;Ruch, JV;Butler, WT
牙本质唾液蛋白（DSP）和牙本质磷蛋白（DPP）由分化和完全分化的成熟成牙本质细胞独特表达。 DSP 和 DPP 很可能积极参与前牙本质向牙本质的转化。为了比较 DPP 和 DSP 的表达模式，我们构建了小鼠 cDNA 探针。 Northern 分析证实了它们在牙胚来源的 RNA 中的特异性表达，并表明探针与相似或相同的多个转录物发生反应。原位杂交表明，DSP 和 DPP 转录本在小鼠成牙本质细胞和前成釉细胞中被独特检测到并共同分布。这些数据以及 DSP 和 DPP 编码序列的相邻定位表明共同的调节机制控制 DSP 和 DPP 表达。牙乳头培养物中使用 TGF β 1 与肝素联合实验诱导成牙本质细胞分化，结果表明这两种分子在体外条件下也是共表达的。
Dentin sialoprotein (DSP) and dentin phosphoproteins (DPP) are uniquely expressed by differentiating and fully differentiated mature odontoblasts. It is likely that DSP and DPP actively participate in the conversion of predentin to dentin. To compare the expression patterns of DPP and DSP, we constructed mouse cDNA probes. Northern analyses confirmed their specific expressions in tooth germ-derived RNA and showed that the probes reacted with similar or identical multiple transcripts. In situ hybridization indicated that DSP and DPP transcripts were uniquely detected and codistributed in developing mouse odontoblasts and preameloblasts. These data, as well as the adjacent positioning of the DSP and DPP coding sequences, suggest that common regulatory mechanisms control DSP and DPP expressions. Dental papillae cultures, in which odontoblast differentiation was experimentally induced with TGF beta 1 combined with heparin, were used to show that the two molecules are also coexpressed under in vitro conditions.