Commonalities and differences between Brassica and Arabidopsis self-incompatibility.
Commonalities and differences between Brassica and Arabidopsis self-incompatibility.
复制标题
DOI：
10.1038/hortres.2014.54
复制
发表时间：
2014
影响因子：
8.7
通讯作者：
Nishio T
中科院分区：
文献类型：
--
作者：
Yamamoto M;Nishio T
在高等植物中，自交不亲和机制对于抑制自交受精和促进异交具有重要意义。在十字花科植物中，自交不亲和反应是由柱头定位的S位点受体激酶(SRK)与花粉毛被定位的配体(Scr/Sp11)的等位基因特异性相互作用所介导的。所分析的所有自交不亲和十字花科植物都在S基因座区域存在SRK和Scr/SP11基因。虽然拟南芥是自交不亲和的，但从自交不亲和的拟南芥中转化SRK-SCR功能基因可以赋予拟南芥自交不亲和的表型。SRK和SCR之间的等位基因特异性相互作用激活了自交不亲和的下游信号级联反应。以SRK的激酶域为诱饵的酵母双杂交分析和遗传分析揭示了甘蓝型油菜自交不亲和信号的几个候选成分。最近，利用自交不亲和转基因甘蓝型油菜植株对已鉴定的甘蓝型油菜自交不亲和信号基因的同源基因进行了鉴定，发现这些同源基因在转基因甘蓝型油菜中不参与自交不亲和信号的传递。本文综述了甘蓝型油菜和拟南芥在S基因座基因组区域和自交不亲和信号转导方面的共性和差异性。
In higher plants, the self-incompatibility mechanism is important for inhibition of self-fertilization and facilitation of out-crossing. In Brassicaceae, the self-incompatibility response is mediated by allele-specific interaction of the stigma-localized S-locus receptor kinase (SRK) with the pollen coat-localized ligand (SCR/SP11). All self-incompatible Brassicaceae plants analyzed have been found to have the SRK and SCR/SP11 genes in the S-locus region. Although Arabidopsis thaliana is self-compatible, transformation with functional SRK-SCR genes from self-incompatible Arabidopsis species confers the self-incompatibility phenotype to A. thaliana. The allele-specific interaction between SRK and SCR activates the downstream signaling cascade of self-incompatibility. Yeast two-hybrid analysis with a kinase domain of SRK as bait and genetic analysis suggested several candidate components of self-incompatibility signaling in Brassica. Recently, A. thaliana genes orthologous to the identified genes for Brassica self-incompatibility signaling were evaluated by using a self-incompatible transgenic A. thaliana plant and these orthologous genes were found not to be involved in self-incompatibility signaling in the transgenic A. thaliana. In this review, we describe common and different aspects of S-locus genomic regions and self-incompatibility signaling between Brassica and Arabidopsis.