Differences in gene expression between noninvasive and invasive transitional cell carcinoma of the human bladder using complementary deoxyribonucleic acid microarray: Preliminary results
Differences in gene expression between noninvasive and invasive transitional cell carcinoma of the human bladder using complementary deoxyribonucleic acid microarray: Preliminary results
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DOI：
10.1016/j.urolonc.2005.07.011
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发表时间：
2006-03-01
影响因子：
2.7
通讯作者：
Kanayama, H
中科院分区：
文献类型：
--
作者：
Elsamman, E;Fukumori, T;Kanayama, H
本研究旨在探讨浅表性非侵袭性和浸润性膀胱移行细胞癌(TCC)基因表达的差异。我们利用含有14551个不同基因的互补脱氧核糖核酸芯片分析了6例浅表性和6例浸润性膀胱移行细胞癌的基因表达，以确定基因表达的差异，这可能解释了不同组织亚型膀胱移行细胞癌生物学和临床预后的差异。对选定的基因进行实时定量聚合酶链式反应，以验证微阵列数据。40个基因的显著上调与浅表性非侵袭性膀胱移行细胞癌有关，但与侵袭性膀胱移行细胞癌无关。这种影响包括与上皮细胞去分化和角质化有关的基因，以及与细胞周期、细胞黏附、转录和凋亡相关的基因。相反，34个基因的显著上调与侵袭性TCC有关，但与病理性浅表性TCC无关，包括与细胞外基质降解、免疫反应、细胞周期和血管生成相关的基因。本研究显示互补脱氧核糖核酸微阵列技术在鉴定不同组织类型膀胱癌基因表达差异方面的有效性。(C)2006 Elsevier Inc.保留所有权利。
This study was performed to identify differences in gene expression between superficial noninvasive and invasive transitional cell carcinoma (TCC) of the bladder in human beings. We used complementary deoxyribonucleic acid rnicroarrays containing 14,551 different genes to analyze gene expression among 6 cases of superficial and 6 cases of invasive TCC of the bladder to identify differences in gene expression, which might explain differences in the biology and clinical outcomes of these histologic subtype of TCC. Quantitative real-time polymerase chain reaction was performed for selected genes to validate the microarray data. Significant up-regulation of 40 genes was associated with cases of superficial noninvasive, but not in invasive, TCC of the urinary bladder. This effect included genes involved in epithelial cell dedifferentiation and keratinization, as well as genes related to cell cycle, cell adhesion, transcription, and apoptosis. Conversely, significant up-regulation of 34 genes was associated with cases of invasive TCC, but not ill superficial TCC, including genes related to extracellular matrix degradation, immune responses, cell cycling, and angiogenesis. This study shows the usefulness of complementary deoxyribonucleic acid microarray technology for identifying differences in gene expression anion-different histotypes of bladder cancer. (c) 2006 Elsevier Inc. All rights reserved.