Anti-Inflammatory Effect of Apigenin on LPS-Induced Pro-Inflammatory Mediators and AP-1 Factors in Human Lung Epithelial Cells
Anti-Inflammatory Effect of Apigenin on LPS-Induced Pro-Inflammatory Mediators and AP-1 Factors in Human Lung Epithelial Cells
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DOI：
10.1007/s10753-015-0232-z
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发表时间：
2016-02-01
期刊：
影响因子：
5.1
通讯作者：
Sharma, S. Chidananda
中科院分区：
文献类型：
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作者：
Patil, Rajeshwari H.;Babu, R. L.;Sharma, S. Chidananda
芹菜素是水果和蔬菜中存在的植物黄酮类化合物之一，是重要的营养保健成分。它被认为是一种潜在的抗氧化剂、抗菌剂和抗炎分子。在本研究中，研究了芹菜素对人肺A549细胞中脂多糖（LPS）诱导的促炎细胞因子和激活蛋白1（AP-1）因子的抗炎作用机制。通过分析促炎细胞因子、一氧化氮合酶 (iNOS)、环氧合酶-2 (COX-2) 和不同 AP-1 因子的表达，确定芹菜素对 LPS 诱导的炎症的抗欧洲炎症活性。芹菜素显着抑制 LPS 诱导的 iNOS、COX-2 表达、促炎细胞因子（IL-1β、IL-2、IL-6、IL-8 和 TNF-α）和 AP-1 蛋白（c-Jun、c-Fos 和 JunB）的表达，包括一氧化氮的产生。研究证实芹菜素通过抑制参与炎症的炎症介质和AP-1因子的表达而发挥抗炎作用，及其在肺部炎症性疾病治疗中的重要性。
Apigenin is one of the plant flavonoids present in fruits and vegetables, acting as an important nutraceutical component. It is recognized as a potential antioxidant, antimicrobial, and anti-inflammatory molecule. In the present study, the mechanism of anti-inflammatory action of apigenin on lipopolysaccharide (LPS)-induced pro-inflammatory cytokines and activator protein-1 (AP-1) factors in human lung A549 cells was investigated. The antiaEuroinflammatory activity of apigenin on LPS-induced inflammation was determined by analyzing the expression of pro-inflammatory cytokines, nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and different AP-1 factors. Apigenin significantly inhibited the LPS-induced expression of iNOS, COX-2, expression of pro-inflammatory cytokines (IL-1 beta, IL-2, IL-6, IL-8, and TNF-alpha), and AP-1 proteins (c-Jun, c-Fos, and JunB) including nitric oxide production. Study confirms the anti-inflammatory effect of apigenin by inhibiting the expression of inflammatory mediators and AP-1 factors involved in the inflammation and its importance in the treatment of lung inflammatory diseases.